葛花枳椇子配伍使用对急性酒精中毒小鼠醉酒症状行为学及脑纹状体和海马神经递质的影响

目的:探讨葛花枳椇子单独或配伍的最佳给药方式及对急性酒精中毒小鼠脑纹状体多巴胺(dopamine,DA)、5-羟色胺(5-hydroxytryptamine,5-HT)含量和海马乙酰胆碱酯酶(acetylcholinesterase,Ach E)活性的影响。方法:选取ICR小鼠186只,随机分为空白对照组、模型组、阳性对照组、葛花组、枳椇子组及配伍1、2、3组。预防给药,给药30 min后灌酒建立模型,2 h后检测脑纹状体DA、5-HT及海马Ach E的含量。结果:葛花枳椇子单用及配伍对急性酒精中毒的预防作用优于治疗作用。急性酒精中毒小鼠脑纹状体5-HT和海马中Ach E含量较空白对照组显著升高(P0.05);与模型组比较,枳椇子组可显著降低纹状体DA含量(P0.05),配伍1,3组可显著降低纹状体5-HT含量(P0.05)。结论:葛花枳椇子单用及配伍对急性酒精中毒的预防作用更优;葛花枳椇子单独或配伍使用可以调节脑组织神经递质,改善急性酒精中毒时的神经行为异常。

Effect of Compatibility of Flos Puerariae Lobatae and Semen Hoveniae on Drunkenness Behavioral and Neurotransmitters of Hippocampus and Striatum in Acute Alcoholism Mice

To investigate better administration method of compatibility of Flos Puerariae Lobatae and Semen Hoveniae, and the influence on content of striatum dopamine (DA), 5-hydroxytryptamine (5-HT) and activity of hippocampus acetylcholinesterase (AchE). Methods:ICR mice were randomly divided into eight groups, including blank control group, model group, positive control group, Gehua group, Zhijuzi group, and combination group 1, 2, 3. The mouse model of acute alcoholism was established by giving alcohol to mice orally after 30 min for taking preventive administration method. The striatum DA, 5-HT and hippocampus AchE were measured after 2 h. Results:The compatibility of Flos Puerariae Lobatae and Semen Hoveniae was superior by prevention to treatment. The contents of striatum 5-HT and activity of achippocampus AchE in the model group were increased compared with those in the blank control group (P<0.05). The contents of DA were obviously reduced in Zhijuzi groups (P<0.05). The contents of 5-HT were significantly decrease in combination group 1, 3 (P<0.05). Conclusion:There is an improvement on neurobehavioral abnormality by regulating the contents of DA, 5-HT and activity of AchE of compatibility of Flos Puerariae Lobatae and Semen Hoveniae.