祛瘀化痰方联合促排卵方案对多囊卵巢综合征患者胰岛素信号通路相关蛋白的影响

目的:探讨祛瘀化痰方联合促排卵方案对多囊卵巢综合征(PCOS)患者胰岛素信号通路相关蛋白的影响。方法:选取2016年4月至2018年4月青海大学附属医院收治的PCOS患者100例,按照随机数字表法随机分为对照组和观察组,每组50例,对照组行氯米芬(CC)+人绝经期促性腺激素(h MG)促排卵方案治疗,观察组在对照组治疗的基础上联合中药祛瘀化痰方治疗,比较2组患者临床疗效、中医证候积分及胰岛素信号通路相关蛋白的表达。结果:治疗后,观察组治疗总有效率较对照组高,差异有统计学意义(P 0. 05); 2组中医证候积分与治疗前比较,差异无统计学意义(P 0. 05);与治疗前比较,治疗后2组中医证候积分明显降低,对照组高于观察组,差异有统计学意义(P 0. 05); 2组治疗后的AKT蛋白表达比治疗前均有明显升高,且观察组上述指标改善情况较对照组明显,差异有统计学意义(P 0. 05); 2组治疗后的P-INSR、INSR、P-IRS、IRS、P-PI3K、PI3K、P-AKT、P-ERK1、ERK1、P-ERK2、ERK2蛋白表达比治疗前均有明显下降,且观察组上述指标改善较对照组明显,差异有统计学意义(P 0. 05)。结论:对PCOS患者实施祛瘀化痰方剂联合西药治疗效果显著,能有效减轻患者临床症状,调控机体胰岛素信号通路相关蛋白的表达。

Effects of Quyu Huatan Decoction Combined with Ovulation Promotion Scheme on Proteins Related to Insulin Signaling Pathway in Patients with Polycystic Ovarian Syndrome

To investigate the effects of Quyu Huatan Decoction combined with ovulation promotion scheme on proteins related to insulin signaling pathway in patients with polycystic ovarian syndrome (PCOS).Methods:A total of 100 PCOS patients in Affiliated Hospital of Qinghai University from April 2016 to April 2018 were divided into two groups (control group and observation group) by random number table,with 50 cases in each group.The control group was given clomiphene citrate (CC) and human menopausal gonadotropin (hMG) ovulation promotion scheme,while the observation group was treated with Quyu Huatan Decoction based on the same treatment of the control group.The clinical efficacy,symptom scores of Tradition Chinese Medicine (TCM),expression of proteins related to insulin signaling pathway were compared between the two groups.Results:After treatment,the total effective rate of the observation group was higher than that of the control group (P<0.05).The TCM symptom scores of two groups were compared with those before treatment,and there was no statistical difference (P>0.05).Compared with before treatment,the TCM symptom scores of two groups decreased after treatment,and the score of control group was higher than that of the observation group (P<0.05).After treatment,the AKT protein expression levels of two groups increased compared with those before treatment,and the improvement of AKT protein expression in the observation group was better than that in the control group (P<0.05).After treatment,the P-INSR,INSR,P-IRS,IRS,P-PI3K,PI3K,P-AKT,P-ERK1,ERK1,P-ERK2,ERK2 protein expression levels in two groups decreased compared with those before treatment,and the improvement of above-mentioned indexes in the observation group was better than that in the control group (P<0.05).Conclusion:Quyu Huatan Decoction combined with western medicine in treatment of PCOS is effective,which can improve clinical symptoms and regulate the expression of proteins related to insulin signaling pathway.