| 肠胃清抑制化疗诱导的小鼠结肠癌肺转移的实验研究 |
| |
| 引用本文： | 吴瑞影,张勇,梁芳,陈彬,万光升,梁梦婷,许建华. 肠胃清抑制化疗诱导的小鼠结肠癌肺转移的实验研究[J]. 上海中医药杂志, 2018, 52(6): 62-66 |
| |
| 作者姓名： | 吴瑞影 张勇 梁芳 陈彬 万光升 梁梦婷 许建华 |
| |
| 作者单位： | 上海中医药大学附属普陀医院肿瘤科 |
| |
| 基金项目： | 国家自然科学基金资助项目(81573940)；上海市普陀区卫生系统自主创新科研资助项目(2015) |
| |
| 摘 要： | 目的研究肠胃清对化疗诱导的小鼠结肠癌肺转移的抑制作用并探讨其机制。方法通过一次性腹腔注射最大耐受剂量(MTD)奥沙利铂,3天后尾静脉注射结肠癌细胞CT26,建立化疗诱导的小鼠结肠癌肺转移模型,24只BALB/c小鼠随机分为模型组、化疗诱导肺转移组(L-OHP)、肠胃清加化疗诱导肺转移组(CWQ+L-OHP)。统计肺结节数目,苏木素-伊红染色(HE)检测肺组织内瘤灶,免疫组化检测肺转移瘤组织微血管密度(MVD);蛋白免疫印迹(Western blot)、实时荧光定量PCR(qRT-PCR)检测肺转移灶血管内皮生长因子(VEGF-A)、基质金属蛋白酶2/9(MMP2/9)蛋白和mRNA的表达。结果与L-OHP组比较,CWQ+L-OHP组肺转移结节数目减少(P0.05)、肿瘤组织MVD值下降(P0.01),肺组织VEGF-A、MMP2、MMP9蛋白和mRNA的表达均下调(P0.01)。结论肠胃清能抑制化疗诱导的小鼠结肠癌肺转移,与其下调肿瘤组织VEGF-A、MMP2、MMP9的表达,降低肿瘤组织MVD,减少肿瘤血管生成有关。
|
| 关 键 词： | 肠胃清 化疗 结肠癌 肺转移 血管内皮生长因子 基质金属蛋白酶 |
| 收稿时间： | 2018-01-08 |
Inhibitory effects of Changweiqing on chemotherapy induced lung metastasis of colon cancer in mice |
| |
| WU Ruiying,ZHANG Yong,LIANG Fang,CHEN Bin,WAN Guangsheng,LIANG Mengting and XU Jianhua. Inhibitory effects of Changweiqing on chemotherapy induced lung metastasis of colon cancer in mice[J]. Shanghai Journal of Traditional Chinese Medicine, 2018, 52(6): 62-66 |
| |
| Authors: | WU Ruiying ZHANG Yong LIANG Fang CHEN Bin WAN Guangsheng LIANG Mengting XU Jianhua |
| |
| Affiliation: | Department of Oncology,Putuo Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200333,China,Department of Oncology,Putuo Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200333,China,Department of Oncology,Putuo Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200333,China,Department of Oncology,Putuo Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200333,China,Department of Oncology,Putuo Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200333,China,Department of Oncology,Putuo Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200333,China and Department of Oncology,Putuo Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200333,China |
| |
| Abstract: | Objective:To study the inhibitory effects of Changweiqing on chemotherapy induced lung metastasis of colon cancer in mice and explore its mechanism. MethodsThe chemotherapy induced lung metastasis of colon cancer model was established by one-time intraperitoneal injection of oxaliplatin(L-OHP)at maximum tolerable dose and intravenous injection of colon cancer CT26 cells via caudal vein 3 days later. 24 BALB/c mice were randomly divided into the model group,chemotherapy induced lung metastasis(L-OHP)group,Changweiqing plus chemotherapy induced lung metastasis(CWQ+L-OHP)group. The nodules number of lung metastasis was counted,the tumor lesions in the lung were detected by HE staining,the microvessel density(MVD)of lung metastatic tumor tissue was detected by immunohistochemistry,and the protein and mRNA expressions of vascular endothelial growth factor(VEGF)-A,matrix metalloproteinase 2/9(MMP2/9)in the lung metastasis were detected by Western blot and qRT-PCR. Results:Compared with the L-OHP group,the nodules number of lung metastasis was reduced in the CWQ+L-OHP group(P<0.05),and the MVD value of tumor tissue was decreased(P<0.01),and the protein and mRNA expressions of VEGF-A,MMP2 and MMP9 in the lung tissue were all down-regulated(P<0.01). Conclusion:Changweiqing can inhibit chemotherapy induced lung metastasis of colon cancer in mice. Its mechanism may be related to down-regulating the expressions of VEGF-A,MMP2 and MMP9 in tumor tissue and reducing MVD and tumor angiogenesis. |
| |
| Keywords: | Changweiqing chemotherapy colon cancer lung metastasis vascular endothelial growth factor matrix metalloproteinase |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《上海中医药杂志》浏览原始摘要信息 |
|
点击此处可从《上海中医药杂志》下载免费的PDF全文 |
|
