基于iTRAQ技术的慢性心力衰竭气虚血瘀证及气阴两虚证组差异蛋白质组学研究

目的:寻找慢性心力衰竭(CHF)(Chronic Heart Failure,CHF)气虚血瘀证及气阴两虚证的相关蛋白质组学特征,探讨证候发生发展机制的可能生物学通路。方法:研究设计分为CHF气虚血瘀证、气阴两虚证、健康对照组,利用i TRAQ标记定量结合串联质谱技术分析CHF气虚血瘀证及气阴两虚证的相关表达差异蛋白质,为蛋白质组学层面的疾病与证候生物学基础提供实验依据,可以在一定层面上揭示证的内涵。结果:与健康对照组比较,在CHF气虚血瘀证组表达差异蛋白质有16个,其中载脂蛋白E、半乳糖凝集素-3结合蛋白等11个蛋白质表达上调,维生素D结合蛋白、胰蛋白酶抑制剂等5个蛋白质表达下调;与健康对照组比较,在CHF气阴两虚证组表达差异的蛋白质有15个,其中补体9、间-α-胰蛋白酶抑制剂家族重链相关蛋白等10个蛋白质表达上调,前血清淀粉样蛋白P、维生素D结合蛋白等5个蛋白质表达下调。结论:蛋白质组检测结果能够反映CHF气虚血瘀证及气阴两虚证的生物学基础,从而能客观的评价证候特征,为病证结合的研究开拓了新的视野。

Differential Proteomics Study of Chronic Heart Failure with Qi Deficiency and Blood Stasis Syndrome and Qi and Yin Deficiency Syndrome Based on iTRAQ Technology

To explore proteomics characteristics of chronic heart failure (CHF) with qi deficiency and blood stasis syndrome and qi and yin deficiency syndrome and possible biological pathways of the mechanism.Methods:This study was designed to divide the participants into CHF with qi deficiency and blood stasis syndrome group,qi and yin deficiency syndrome group,healthy control group.Quantitative iTRAQ marker as a new quantitative proteomics technology combined with tandem mass spectrometry was used to analyze the differential protein expression of CHF with qi deficiency and blood stasis syndrome group and qi and yin deficiency syndrome,which may provide experimental evidence for the disease and biological basis in protein level,and reveal the content of syndrome in a certain level.Results:Compared with healthy control group,a total of 16 kinds of protein had differential expression in qi deficiency and blood stasis group.The expressions of apolipoprotein E,galectin-3-binding protein and other 9 kinds of proteins were up-regulated.The expressions of vitamin D-binding protein (DBP),trypsin inhibitor and other 3 proteins were down-regulated.Compared with health control group,there were 15 kinds of proteins which had differential expressions in qi and yin deficiency group,and complement 9,inter-alpha-trypsin inhibitor family heavy chain-related protein (IHRP) and other 8 proteins expressions were up-regulated.The expressions of pre-serum amyloid P component,DBP and other 3 proteins were down-regulated.Conclusion:Proteomic detection results can reflect the biological basis of CHF qi deficiency and blood stasis syndrome,qi and yin deficiency syndrome factor,which can objectively evaluate characteristics of this syndrome and provide new view of combined research of disease and syndrome.