松果菊苷对醋酸铅致生精功能损伤小鼠的治疗作用

目的探讨松果菊苷对醋酸铅致生精功能损伤小鼠的治疗作用。方法 60只雄性BALB/c小鼠以单纯随机抽样法分为对照组,模型组,松果菊苷低、中、高剂量组及阳性药物组,每组10只。除对照组外,其余5组小鼠每天腹腔注射醋酸铅20 mg·kg-1·d-1,连续7 d,制备小鼠生殖功能损伤模型。模型制备后,阳性药物组给予人绒毛膜促性腺激素(HCG)500 U·kg-1·d-1腹腔注射,松果菊苷低、中、高剂量组分别给予25 mg·kg-1、50 mg·kg-1、100 mg·kg-1松果菊苷灌胃,对照组和模型组给予等体积0.9%Na Cl溶液灌胃,连续30天;末次灌胃后24 h,分离附睾,观察精子密度、精子活率及精子畸形率;固定睾丸组织,观察组织病理改变;分离血清及匀浆睾丸组织,利用酶联免疫法检测睾丸组织睾酮(T)及血清黄体生成素(LH)、卵泡刺激素(FSH)。结果与对照组比较,模型组精子密度、精子活率降低,精子畸形率升高,睾丸组织匀浆中T含量降低,血清FSH和LH水平升高,差异均有统计学意义(P0.01)。与模型组比较,松果菊苷各剂量组精子密度、精子活率升高,精子畸形率降低,血清FSH和LH水平降低,睾丸组织匀浆中T含量升高,差异均有统计学意义(P0.01),且松果菊苷对生殖功能损伤的治疗作用呈剂量依赖性。组织学观察显示,松果菊苷各剂量组小鼠睾丸组织病理性损害均得到改善。结论松果菊苷对醋酸铅致生精障碍小鼠生精功能有明显的治疗作用,其作用机制可能与改善生殖激素水平,恢复下丘脑-垂体-性腺轴的调控功能有关。

Therapeutic effects of echinacoside on lead acetate induced dyszoospermia in mice

  Objective:To discuss the therapeutic effects of echinacoside on lead acetate induced dyszoospermia in mice. Methods60 male BALB/c mice were randomly divided into the control group,model group,positive drug group and echinacoside groups with low-,middle- and high-dose,10 mice in each group. Except the control group,the mouse model of dyszoospermia was established by intraperitoneal injection of lead acetate at daily dose of 20 mg/kg for continuous 7 days in the other groups. After successful modeling,the positive drug group was treated with human chorionic gonadotropin(HCG)by intraperitoneal injection at daily dose of 500 U/kg. The echinacoside groups with low-,middle- and high-dose were treated with echinacoside by intragastric administration at doses of 25 mg/kg,50 mg/kg and 100 mg /kg,respectively. The control group and the model group were treated with 0.9% NaCl solution by intragastric administration at equivalent volume,with a course of 30 days. 24 hours after the last intragastric administration,the epididymis was separated,the sperm density,sperm motility rate and teratospermia rate were observed. The testicular tissue was fixed to observe the histopathological changes. The serum was separated and the testicular tissue was homogenized,the level of testosterone(T)in testicular tissue and the levels of luteinizing hormone(LH)and follicle stimulating hormone(FSH)in serum were detected by enzyme linked immunosorbent assay.  Results:Compared with the control group,the sperm density and the sperm motility rate were decreased and the teratospermia rate was increased in the model group,and the T level in the testicular tissue homogenate was decreased and the levels of FSH and LH in serum were increased,with statistically significant differences(P<0.01). Compared with the model group,the sperm density and the sperm motility rate were increased and the teratospermia rate was decreased in the echinacoside groups with different doses,and the levels of FSH and LH in serum were decreased and the T level in the testicular tissue homogenate was increased,with statistically significant differences(P<0.01). The therapeutic effects of echinacoside on the reproductive impairment were in a dose-dependent manner. The histological observation showed that the pathological injuries in the testicular tissue were improved in echinacoside groups with different doses.  Conclusion:Echinacoside shows obvious therapeutic effects on lead acetate induced dyszoospermia in mice,and its mechanisms may be associated with improving the reproductive hormone levels and recovering the regulation function of hypothalamus-pituitary-gonad axis.