| 护场理论指导下的箍围法对皮肤脓肿大鼠PCT、CRP、MMP-2及不同位置组织病理的影响 |
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| 引用本文： | 徐强,王婉莹,刘婷婷,刘现周,朱朝军,张朝晖.护场理论指导下的箍围法对皮肤脓肿大鼠PCT、CRP、MMP-2及不同位置组织病理的影响[J].上海中医药杂志,2018,52(6):78-82, 94. |
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| 作者姓名： | 徐强 王婉莹 刘婷婷 刘现周 朱朝军 张朝晖 |
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| 作者单位： | 天津中医药大学第二附属医院中医外科;天津中医药大学研究生院 |
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| 基金项目： | 国家自然科学基金面上项目(81273759) |
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| 摘 要： | 目的观察护场理论指导下的箍围药红肿消酊外用对皮肤脓肿大鼠血浆C反应蛋白(CRP)、降钙素原(PCT)、分泌物基质金属蛋白酶-2(MMP-2)以及脓肿周围不同位置组织病理的影响。方法将40只SD大鼠分为空白组、预治疗组、治疗组、对照组、模型组,除空白组外,其余4组皮下注射金黄色葡萄球菌造成皮下脓肿模型,采用不同药物换药治疗。分别于治疗3、7、14 d每组大鼠行心脏取血1.5 ml,同时采用100μl 0.9%Na Cl溶液冲洗除空白组外的大鼠创面后回收伤口分泌物,酶联免疫吸附(ELISA)法检测血浆内CRP、PCT以及分泌物中MMP-2的含量,14 d后处死大鼠,提取大鼠脓肿周围1 cm、2 cm、3 cm皮下组织制成切片,Masson染色,镜下观察大鼠脓肿周围不同位置皮肤及肌肉组织炎细胞浸润、血管增生及毛细血管扩张情况。结果治疗期间模型组、对照组、预治疗组及治疗组在各时期血浆CRP及PCT含量均呈下降趋势且高于空白组,并在治疗末期趋于稳定。在治疗期间,采用红肿消酊剂外用的预治疗组及治疗组血浆CRP及PCT含量均不同程度低于对照组及模型组,在治疗早期(3 d、7 d)时采用莫匹罗星软膏外用的对照组血浆CRP及PCT含量与预治疗组及治疗组相比差异有统计学意义(P0.05,P0.01),在治疗14 d时预治疗组及治疗组血浆CRP及PCT含量与对照组接近,差异无统计学意义(P0.05)。在治疗中后期(7 d、14 d)时,预治疗组及治疗组创面分泌物MMP-2含量低于模型组与对照组,差异有统计学意义(P0.05,P0.01)。镜下观察脓肿周围组织显示治疗组及预治疗组距离脓肿不同位置皮下肌肉组织炎细胞浸润显著低于对照组及模型组。结论应用红肿消酊箍围治疗大鼠皮肤脓肿,能够通过降低血浆内CRP及PCT含量,同时能够降低分泌物MMP-2含量,减轻脓肿周围组织炎性浸润,改善全身及局部感染,从而为护场形成提供有利条件。
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| 关 键 词： | 护场理论 箍围法 脓肿 炎性因子 大鼠 |
| 收稿时间： | 2018/2/24 0:00:00 |
Effects of therapy of encircling lesion with drugs under guidance of defended field theory on levels of PCT,CRP,MMP-2 and histopathology at different locations in rats with skin abscess |
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| XU Qiang,WANG Wanying,LIU Tingting,LIU Xianzhou,ZHU Chaojun and ZHANG Zhaohui.Effects of therapy of encircling lesion with drugs under guidance of defended field theory on levels of PCT,CRP,MMP-2 and histopathology at different locations in rats with skin abscess[J].Shanghai Journal of Traditional Chinese Medicine,2018,52(6):78-82, 94. |
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| Authors: | XU Qiang WANG Wanying LIU Tingting LIU Xianzhou ZHU Chaojun and ZHANG Zhaohui |
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| Institution: | Department of Chinese Medical Surgery,Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300250,China,Graduate School,Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China,Graduate School,Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China,Department of Chinese Medical Surgery,Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300250,China,Department of Chinese Medical Surgery,Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300250,China and Department of Chinese Medical Surgery,Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300250,China |
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| Abstract: | |
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| Keywords: | defended field theory therapy of encircling lesion with drugs abscess inflammatory factors rat |
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