芍药苷和芍药内酯苷的抗抑郁作用与NO/cGMP信号转导通路的相关性

目的:比较赤芍、白芍及其有效成分芍药苷、芍药内酯苷对小鼠的抗抑郁作用及其与NO/c GMP信号转导通路的相关性。方法:选取小鼠悬尾实验模型,给予赤芍水提物、白芍水提物,记录小鼠悬尾不动时间。采用相同实验方法给予小鼠芍药苷和芍药内酯苷,并检测小鼠大脑皮质及海马组织中NO/c GMP通路相关指标。结果:与模型组比较,2 g/kg赤芍和2 g/kg、1 g/kg白芍组小鼠悬尾不动时间减少,差异有统计学意义(P0.05)。与2 g/kg赤芍同剂量组比较,白芍的小鼠悬尾不动时间减少,差异有统计学意义(P0.05)。与模型组比较,20 mg/kg芍药苷组、20 mg/kg芍药内酯苷组的小鼠悬尾不动时间及NO、c GMP含量明显减少,差异有统计学意义(P0.05);且与10 mg/kg芍药苷同剂量组比较,芍药内酯苷组的小鼠悬尾不动时间及NO含量明显减少,差异有统计学意义(P0.05)。与模型组比较,20 mg/kg芍药苷组及20mg/kg芍药内酯苷组的小鼠n NOS、GluR1mRNA表达明显减少,差异有统计学意义(P0.05)。结论:白芍具有抗抑郁作用,芍药苷和芍药内酯苷是白芍抗抑郁作用的物质基础;与白芍比较,赤芍的抗抑郁作用较弱,与其主要含有抗抑郁作用较弱的芍药苷,而不含抗抑郁作用较强的芍药内酯苷有关。

Correlation Study on Antidepressant Effect of Paeoniflorin and Albiflorin and NO/cGMP Signal Transduction Pathway

To investigate the correlation between antidepressant effects of paeoniflorin and albiflorin on mice and NO/cGMP signal transduction pathway. Methods:Radix Paeoniae Rubra and Radix Paeoniae Alba was intragastric administered to the mice to establish the mice model of antidepressant with tail suspension test (TST), and the time of immobility was recorded. The same methods were used to study paeoniflorin and albiflorin. And the NO/cGMP pathway related indicators of cerebral cortex and hippocampus were detected. Results:Compared with model group, 2 g/kg Radix Paeoniae Rubra and 2 g/kg, 1 g/kg Radix Paeoniae Alba can reduce the immobility time of tail suspension (P<0.05, P<0.001, P<0.05). Compared with the same dose of Radix Paeoniae Rubra, the TST immobility time of 2 g/kg Radix Paeoniae Alba were obviously lower (P<0.05). Compared with model group, 20 mg/kg paeoniflorin, 20 mg/kg albiflorin can reduce the immobility time of tail suspension and the content of NO, cGMP (P<0.05, P<0.001); Compared with the same dose of paeoniflorin, the immobility time of tail suspension and the content of NO of 10 mg/kg albiflorin were obviously lower (P<0.05). Compared with model group, 20 mg/kg paeoniflorin and 20 mg/kg albiflorin can reduce the mRNA expression of nNOS, GluR1 (P<0.05, P<0.01, P<0.05). Conclusion:Radix Paeoniae Alba has antidepressant effect, and paeoniflorin and albiflorin are the material basis of the antidepressant effect of Radix Paeoniae Alba; however, in the administration range of this experiment, the antidepressant effect of Radix Paeoniae Rubra is not obvious compared with that of Radix Paeoniae Alba, which may be related with it mainly containing paeoniflorin which antidepressant effect is weaker, but not containing albiflorin which antidepressant effect is stronger.