| 铁皮石斛蛋白磷酸酶2A基因的分子克隆研究 |
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| 引用本文： | 刘亮亮,张娜,黑小斌,李欢,李元敏,李佳慧,孙涛,张岗.铁皮石斛蛋白磷酸酶2A基因的分子克隆研究[J].上海中医药杂志,2018,52(9):61-64, 68. |
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| 作者姓名： | 刘亮亮 张娜 黑小斌 李欢 李元敏 李佳慧 孙涛 张岗 |
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| 作者单位： | 陕西中医药大学药学院;陕西省中药基础与新药研究重点实验室 |
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| 基金项目： | 陕西省高校青年杰出人才支持计划项目；陕西省咸阳市中青年科技领军人才项目 |
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| 摘 要： | 目的克隆铁皮石斛蛋白磷酸酶(protein phosphatase)2A基因,进行生物信息及表达特征分析。方法运用RACE克隆基因,生物信息学软件进行核酸及蛋白质理化性质、保守结构域、亚细胞定位等分析;用DNAStar 7.0、MEGA 6.0分别进行蛋白序列比对和进化分析。借助定量PCR检测基因表达模式。结果 Do PP2A(Gen Bank注册号KT957552)c DNA 1 410 bp,与其他植物PP2A同源性为89%~94%。ORF编码一条由306个氨基酸组成的肽链,分子量35.19 k D,等电点4.79。推导蛋白含有金属依赖性磷酸酶保守结构域,蛋白不含信号肽或跨膜残基,定位于细胞质。Do PP2A与其他植物的PP2A相比具有高度的保守性,聚在PP2A分子进化树的A组。基因转录本在石斛根和茎中分别为叶中的4.41倍和1.45倍。结论 Do PP2A分子克隆及特征为研究其在铁皮石斛生长发育中的分子功能提供基础。
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| 关 键 词： | 铁皮石斛 蛋白磷酸酶 克隆 生物信息学 表达 |
| 收稿时间： | 2018/5/22 0:00:00 |
Molecular cloning of protein phosphatase 2A gene in Dendrobium officinale |
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| LIU Liangliang,ZHANG N,HEI Xiaobin,LI Huan,LI Yuanmin,LI Jiahui,SUN Tao and ZHANG Gang.Molecular cloning of protein phosphatase 2A gene in Dendrobium officinale[J].Shanghai Journal of Traditional Chinese Medicine,2018,52(9):61-64, 68. |
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| Authors: | LIU Liangliang ZHANG N HEI Xiaobin LI Huan LI Yuanmin LI Jiahui SUN Tao and ZHANG Gang |
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| Institution: | College of Pharmacy, Shaanxi University of Chinese Medicine; Shaanxi Provincial Key Laboratory for Chinese Medicine Basis & New Drugs Research, Xianyang 712046, China,College of Pharmacy, Shaanxi University of Chinese Medicine; Shaanxi Provincial Key Laboratory for Chinese Medicine Basis & New Drugs Research, Xianyang 712046, China,College of Pharmacy, Shaanxi University of Chinese Medicine; Shaanxi Provincial Key Laboratory for Chinese Medicine Basis & New Drugs Research, Xianyang 712046, China,College of Pharmacy, Shaanxi University of Chinese Medicine; Shaanxi Provincial Key Laboratory for Chinese Medicine Basis & New Drugs Research, Xianyang 712046, China,College of Pharmacy, Shaanxi University of Chinese Medicine; Shaanxi Provincial Key Laboratory for Chinese Medicine Basis & New Drugs Research, Xianyang 712046, China,College of Pharmacy, Shaanxi University of Chinese Medicine; Shaanxi Provincial Key Laboratory for Chinese Medicine Basis & New Drugs Research, Xianyang 712046, China,College of Pharmacy, Shaanxi University of Chinese Medicine; Shaanxi Provincial Key Laboratory for Chinese Medicine Basis & New Drugs Research, Xianyang 712046, China and College of Pharmacy, Shaanxi University of Chinese Medicine; Shaanxi Provincial Key Laboratory for Chinese Medicine Basis & New Drugs Research, Xianyang 712046, China |
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| Abstract: | |
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| Keywords: | Dendrobium officinale protein phosphatase clone bioinformatics expression |
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