| 鼓槌石斛乙醇提取物对糖尿病大鼠视网膜组织凋亡相关因子表达的影响 |
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| 引用本文： | 高玮,李春霞,柯尊记,刘芳,秦瑜,陆骏. 鼓槌石斛乙醇提取物对糖尿病大鼠视网膜组织凋亡相关因子表达的影响[J]. 上海中医药大学学报, 2018, 32(4): 89-94 |
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| 作者姓名： | 高玮 李春霞 柯尊记 刘芳 秦瑜 陆骏 |
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| 作者单位： | 上海中医药大学附属上海市中西医结合医院眼科;上海中医药大学生物化学教研室神经科学研究中心上海市健康辨识与评估重点实验室 |
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| 基金项目： | 上海市卫计委科研基金项目(201640245) |
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| 摘 要： | 目的:观察鼓槌石斛乙醇提取物(EDC)对糖尿病大鼠视网膜凋亡相关因子表达的影响。方法:SD大鼠随机分为正常对照组、糖尿病模型组(DM组)及EDC低剂量组(LEDC组)和高剂量组(HEDC组)。除正常对照组外,其余各组大鼠均通过腹腔注射链脲佐菌素(STZ)建立糖尿病模型。8周后,LEDC组、HEDC组分别给予EDC100 mg·kg~(-1)·d~(-1)、300 mg·kg~(-1)·d~(-1)灌胃,正常对照组和DM组予等量0.9%Na Cl溶液灌胃。干预8周后,采用HE染色观察各组大鼠视网膜各层结构,Western blot、免疫组化法检测视网膜组织Bax、Bcl-2、Caspase-3蛋白表达,TUNEL法观察视网膜组织凋亡指数。结果:与正常对照组比较,DM组视网膜内界膜不完整,内、外核层界限模糊,神经纤维层肿胀明显;而EDC不同剂量组与DM组比较,内、外核层界限渐清晰,神经纤维层肿胀减轻。与正常对照组比较,DM组Bax、Caspase-3蛋白表达增加,Bcl-2蛋白表达下降;LEDC组、HEDC组视网膜Bax、Caspase-3蛋白表达明显低于DM组,Bcl-2蛋白表达明显高于DM组(P0.05)。免疫组化结果显示,正常对照组视网膜中未见Caspase-3阳性表达,DM组Caspase-3明显表达于神经纤维层、内核层、色素上皮层,LEDC、HEDC组少量表达于神经纤维层、色素上皮层,并且HEDC组Caspase-3阳性表达低于LEDC组。TUNEL结果显示,DM组大鼠视网膜细胞凋亡指数明显高于正常对照组(P0.01),而HEDC、LEDC组凋亡指数明显降低(P0.01)。结论:EDC可能通过调控糖尿病视网膜病变过程中凋亡相关因子的表达,对糖尿病大鼠视网膜有一定的改善作用。
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| 关 键 词： | 糖尿病视网膜病变 鼓槌石斛乙醇提取物 细胞凋亡 |
Effects of ethanol extract of Dendrobium chrysotoxum Lindl. on expressions of retinal apoptosis related factors in diabetic rats |
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| GAO Wei,LI Chunxi,KE Zunji,LIU Fang,QIN Yu and LU Jun. Effects of ethanol extract of Dendrobium chrysotoxum Lindl. on expressions of retinal apoptosis related factors in diabetic rats[J]. Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai, 2018, 32(4): 89-94 |
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| Authors: | GAO Wei LI Chunxi KE Zunji LIU Fang QIN Yu LU Jun |
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| Affiliation: | Department of Ophthalmology,Shanghai Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200082,China,Department of Ophthalmology,Shanghai Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200082,China,Department of Biochemistry,Center for Neurosciences,Shanghai Key Laboratory of Health Identification and Assessment,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China,Department of Ophthalmology,Shanghai Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200082,China,Department of Ophthalmology,Shanghai Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200082,China and Department of Ophthalmology,Shanghai Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200082,China |
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| Abstract: | |
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| Keywords: | diabetic retinopathy ethanol extract of Dendrobium chrysotoxum Lindl. apoptosis |
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