| 补肾活血方含药血清对小鼠肢芽细胞增殖和分化的影响 |
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| 引用本文： | 薛纯纯,李晓锋,王利波,王开强,唐德志.补肾活血方含药血清对小鼠肢芽细胞增殖和分化的影响[J].上海中医药杂志,2018,52(5):79-82. |
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| 作者姓名： | 薛纯纯 李晓锋 王利波 王开强 唐德志 |
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| 作者单位： | 上海中医药大学附属市中医医院疼痛科;上海中医药大学附属龙华医院;上海中医药大学脊柱病研究所 |
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| 基金项目： | 国家自然科学基金面上项目(81473701)；国家自然科学基金青年基金项目(81503590)；上海市青年科技英才扬帆计划项目(17YF1417800)；上海中医药大学附属龙华医院龙医学者育苗计划项目(LYTD38)；上海中医药大学杏林青年学者项目(RC20170201) |
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| 摘 要： | 目的探讨补肾活血方对小鼠肢芽细胞向软骨细胞分化增殖的影响。方法分离12.5天小鼠胚胎肢芽细胞进行微团培养,同时用补肾活血方含药血清进行干预。采用MTT法检测肢芽细胞的增殖活性,阿尔新蓝染色检测肢芽细胞分化为软骨细胞能力,RT-PCR法检测肢芽细胞中Col2a1、Col10a1、Mmp9、β-catenin、Runx2 mRNA的表达水平。结果与空白对照组比较,补肾活血方含药血清组在24 h、48 h和72 h 3个时间点均能显著促进小鼠肢芽细胞增殖。微团培养6 d后,阿尔新蓝染色结果显示补肾活血方含药血清组软骨细胞集落形成增加。RT-PCR结果显示补肾活血方含药血清组肢芽细胞Col2a1 mRNA表达水平增加,而Col10a1、Mmp9、β-catenin、Runx2 mRNA表达下调。结论补肾活血方含药血清可能通过β-catenin信号通路促进小鼠胚胎肢芽细胞的增殖与分化。
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| 关 键 词： | 肢芽细胞 微团培养 补肾活血方 软骨形成 |
| 收稿时间： | 2018/1/17 0:00:00 |
Effects of medicated serum of Bushen Huoxue Recipe on proliferation and differentiation of mouse limb bud cells |
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| XUE Chunchun,LI Xiaofeng,WANG Libo,WANG Kaiqiang and TANG Dezhi.Effects of medicated serum of Bushen Huoxue Recipe on proliferation and differentiation of mouse limb bud cells[J].Shanghai Journal of Traditional Chinese Medicine,2018,52(5):79-82. |
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| Authors: | XUE Chunchun LI Xiaofeng WANG Libo WANG Kaiqiang and TANG Dezhi |
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| Institution: | Department of Pain, Shanghai Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China,Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China,Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China,Department of Pain, Shanghai Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China and Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China;Institute of Spine Diseases, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China |
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| Abstract: | |
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| Keywords: | limb bud cell micromass culture Bushen Huoxue Recipe chondrogenesis |
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