| 骨痛灵方调控骨转移模型小鼠脊髓MAPK信号通路的研究 |
| |
| 引用本文： | 王立芳,金贵玉,罗琴琴,吕英,马玥,徐振晔.骨痛灵方调控骨转移模型小鼠脊髓MAPK信号通路的研究[J].上海中医药杂志,2018,52(3):68-71, 76. |
| |
| 作者姓名： | 王立芳 金贵玉 罗琴琴 吕英 马玥 徐振晔 |
| |
| 作者单位： | 上海中医药大学附属龙华医院肿瘤科;上海中医药大学附属岳阳中西医结合医院肿瘤科; |
| |
| 基金项目： | 国家自然科学基金资助项目(81704042);上海市教委科研创新计划资助项目(2017-01-07-00-10-E00064) |
| |
| 摘 要： | 目的从调控MAPK信号通路角度探讨骨痛灵方治疗骨转移疼痛的作用机制。方法将40只小鼠随机分为假手术组、模型组、中药组、对照组,除假手术组外,其余各组建立小鼠骨转移模型。从造模第7天开始给药,中药组予骨痛灵方治疗,对照组以曲马多治疗,采用VonFrey纤维检测机械痛阈值评价其止痛疗效,观察肝肾心病理评价其安全性,采用Western blot法检测脊髓MAPK信号通路中p-JNK、p-p38、p-ERK蛋白的表达。结果 (1)造模第14天,与模型组比较,对照组和中药组机械痛阈值均有升高(P0.05)。造模第21天,中药组机械痛阈值较模型组与对照组均增高(P0.05)。(2)各组心肝肾病理均无明显改变。(3)与假手术组比较,模型组脊髓p-JNK、p-p38、p-ERK的表达均增加(P0.05)。与模型组比较,对照组和中药组脊髓p-JNK、p-p38和p-ERK的表达均显著减少(P0.05)。中药组和对照组脊髓p-JNK、p-p38和p-ERK的表达差异均无统计学意义(P0.05)。结论骨痛灵具有良好的治疗骨转移疼痛的疗效,且对心肝肾无明显毒副作用,其止痛作用机制与调控MAPK信号通路p-JNK、p-p38、p-ERK蛋白表达有关。
|
| 关 键 词： | 骨转移 疼痛 骨痛灵方 MARK信号通路 |
| 收稿时间： | 2017/12/13 0:00:00 |
Regulation of Gutongling Decoction on MAPK signaling pathway in spinal cord of bone metastasis mice |
| |
| WANG Lifang,JIN Guiyu,LUO Qinqin,LYU Ying,MA Yue and XU Zhenye.Regulation of Gutongling Decoction on MAPK signaling pathway in spinal cord of bone metastasis mice[J].Shanghai Journal of Traditional Chinese Medicine,2018,52(3):68-71, 76. |
| |
| Authors: | WANG Lifang JIN Guiyu LUO Qinqin LYU Ying MA Yue and XU Zhenye |
| |
| Institution: | Department of Oncology, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China,Department of Oncology, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China,Department of Oncology, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China,Department of Oncology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China,Department of Oncology, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China and Department of Oncology, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China |
| |
| Abstract: | |
| |
| Keywords: | bone metastasis pain Gutongling Decoction MARK signaling pathway |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《上海中医药杂志》浏览原始摘要信息 |
| 点击此处可从《上海中医药杂志》下载免费的PDF全文 |
|
