| 实时荧光PCR检测手足口病病原体方法的建立及初步应用 |
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| 引用本文： | 唐彦,侯俊,徐军,沈宏辉,陈威巍,李伯安,赵军.实时荧光PCR检测手足口病病原体方法的建立及初步应用[J].中华实验和临床病毒学杂志,2011,25(1):152-154. |
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| 作者姓名： | 唐彦 侯俊 徐军 沈宏辉 陈威巍 李伯安 赵军 |
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| 作者单位： | 解放军军医进修学院,100853;解放军第三○二医院医务部,实验技术保障中心;解放军第三○二医院医务部,临检中心;解放军第三○二医院医务部,感染性疾病治疗中心;解放军第三○二医院医务部,肝衰竭诊疗中心; |
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| 摘 要： | 目的 建立一种能同时检测肠道病毒、肠道病毒71型和柯萨奇病毒16型等手足口病病原体核酸的实时荧光PCR方法.方法 针对上述病毒的基因序列,设计、合成引物、探针.对38例手足口病患者在实时荧光PCR仪上进行扩增、检测和结果分析;并与常规RT-PCR方法的检测结果进行比对.结果 38例患者肠道病毒、肠道病毒71型和柯萨奇病毒16型实时荧光PCR方法与常规RT-PCR方法的阳性率分别为:73.7%、60.5%、13.2%、71.1%、55.3%、13.2%.统计分析表明,两种方法差异不具有统计学意义.结论 成功建立了肠道病毒、肠道病毒71型和柯萨奇16型病毒核酸的实时荧光PCR检测方法.
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| 关 键 词： | 手足口病 肠道病毒属 柯萨奇病毒感染 逆转录聚合酶链反应 |
The establishment of real time fluorescent PCR detecting method and preliminary application on pathogenesis of HFMD |
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| TANG Yan,HOU Jun,XU Jun,SHENG Hong-hui,CHEN Wei-wei,LI Bo-an,ZHAO Jun.The establishment of real time fluorescent PCR detecting method and preliminary application on pathogenesis of HFMD[J].Chinese Journal of Experimental and Clinical Virology,2011,25(1):152-154. |
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| Authors: | TANG Yan HOU Jun XU Jun SHENG Hong-hui CHEN Wei-wei LI Bo-an ZHAO Jun |
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| Abstract: | Objective To establish the real-time fluorescent PCR method for detecting enterovirus,enterovirus 71 and Coxsackievirus A 16 nucleic acid. Methods Primers and MGB probe were chosen for virus gene. The samples of 38 HFMD patients were analyzed by TaqMan-MGB PCR technique on a fluorescence real-time PCR instrument,and the results were compared with those by conventional RT-PCR.Results The real-time fluorescent PCR positive rates of EV, EV71 and Cox AI6 were 73.7% , 60. 5%,13. 2% ;the conventional RT-PCR were 71. 1%, 55.3%, 13.2%. There were no significant differences between the two methods. Conclusion The real-time fluorescent PCR detecting method of EV, EV71 and Cox A16 nucleic acid have been established successfully. |
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