赖氨酰氧化酶对胃癌细胞HGC-27体外迁移黏附能力的影响

Objective To study the effects of lysyl oxidase ( LOX) on the migration and adhesion of the human gastric cancer cell line HGC-27 cells in vitro. Methods The human gastric cancer cell line HGC-27 cells were cultured in vitro, and treated with different concentration of β-aminopropionitrile (BAPN). The ability of migration was assessed by wound-healing assay. The ability of adhesion was detected by homogenous and heterogeneous adhesion experiments. Results Compared that with 0 mmol/L BAPN, the ability of migration of the cells after treatment with 0.2 mmol/L BAPN was descended at 8, 24, 32 and 48 h; the number of cells with homogeneous adhesion was increased from (6.97 ± 0.07) x l03/ml to (7.78±0.11) ×103/ml; and the number of cells with heterogeneous adhesion was decreased from (8.98± 0. 15) x 103/ml to (8. 35 ±0. 10) ×103/ml, both <0. 05. Compared with that of cells treated with 0 mmol/L and 0.2 mmol/L BAPN, the migration ability of cells after treatment with 0.3 mmol/L BAPN was descended at 8, 24, 32 and 48 h; the number of cells with homogeneous adhesion was raised to (8. 02 ± 0. 11) ×103/ml and the number of cells with heterogeneous adhesion was down to (7.93 ±0.07) ×103/ml (P < 0. 05). Conclusion LOX may promote the metastasis of cancer cells by enhancing invasion, increasing heterogeneous adhesion and decreasing homogeneous adhesion.

Effect of lysyl oxidase on migration and adhesion of human gastric cancer HGC-27 cells in vitro