慢加急性肝衰竭中IL-10的表达及其启动子甲基化状态的研究

目的 探讨慢加急性肝衰竭患者血清IL-10的表达及其甲基化状态的意义.方法 慢加急性肝衰竭组25例,慢乙肝组25例,正常对照组10例,ELISA法测定血清IL-10的水平,MSP方法检测IL-10启动子甲基化状态,三组间比较.结果 肝衰竭组、慢乙肝组较正常对照组血清IL-10水平明显升高,有统计学意义(P<0.05);肝衰竭组较慢乙肝组升高,无统计学意义(P>0.05).肝衰竭组IL-10水平与TBIL、MELD明显正相关(分别为r=0.566,r=0.443,P<0.05),与PTA呈明显负相关(r=-0.581,P<0.05),与ALT、HBV-DNA无明显相关性(分别为r=-0.022,r=0.033,P>0.05).肝衰竭组甲基化分布状态较慢乙肝组和正常对照组有统计学差异(P<0.05).结论 肝衰竭、慢乙肝患者IL-10水平明显升高.IL-10水平随肝衰竭的严重程度升高.IL-10启动子区甲基化可能是基因失活的重要机制.

The analysis of IL-10 and its methylation in the patients with acute on chronic liver failure

Objective To investigate the effect of IL-10 and the methylation of its promoter in acute on chronic liver failure (ACLF). Methods Patients were divided into three groups: 25 with ACLF, 25 with CHB, 10 healthy controls. Respectively detect the serum level of IL-10 via ELISA, and the methylation of 1L-10 promoter via MSP,to analyze the difference among the three groups. Results Both the ACLF group and the CHB group have significant increase in serum level of IL-10 compared with the control group ( P <0.05 ) ; the ACLF group's level is higher than the CHB group, however without statistical significance (P >0.05 ). The serum level of IL-10 in ACLF group has no significant relativity with ALT and HBV-DNA( r = -0. 022, r = 0. 033, respectively; P > 0. 05 ) ; has positive relativity with TBIL and MELD ( r = 0. 566, r =0.443, respectively; P < 0.05); and negative relativity with PTA (r = - 0.581, P < 0.05). The distribution of the methylation of IL-10 promoter in ACLF group is significantly different from the other two.Conclusion The serum level of IL-10 in hepatitis patients is significantly higher and increases with the degree of liver failure. The promoter methylation may be important in the gene inactivation.