生长因子网络调节对骨形成作用的研究Ⅱ、IGF—Ⅱ对人胚成骨样细胞增殖及分化的影响

利用体外分离培养人胚成骨样细胞，加入不同剂量胰岛素样生长因子-Ⅱ（IGF-Ⅱ），从时间-效应和剂量一效应两个方面考察IGF-Ⅱ对人胚成骨样细胞增殖与分化的影响，研究结果表明，IGF-Ⅱ的促骨形成效应显著，它对体外培养的人胚成骨细胞DNA、胶原蛋白和碱性磷酸酶 合成均有明显的促进作用，其作用亦呈剂量与效应相依赖的关系，在4个含量组中，10ng/mlIGF-Ⅱ对成骨样细胞的作用最强，IGF-Ⅱ是一种对成骨细胞作用较全面的生长因子，对成骨细胞的增殖和分化均有明显的促进作用。

The effects of IGF-II on the proliferation and differentiation of human osteoblast-like cells]

Biological regulatory mechanism which supports bone cell development within the context of proliferation/differentiation relationship serves as a viable basis for addressing skeletal disorders and provides experiment and theoretic basis for selection and utilization of growth factors to reconstruct bone defect. Osteoblast-like cells isolated from human calvarium were used in this study. IGF-II were investigated by adding in the culture medium with different concentration. The results showed that IGF-II stimulated DNA, collagen synthesis and ALP activity of human osteoblast-like cells. Its effects were strong for proliferation and differentiation of osteoblast cells, when 10 ng/ml of four different concentrations (0.1, 1, 10 and 100 ng/ml) were used. IGF-II was proved to be important factor in bone regeneration.