|
|||||
|
|
| 干预改变细胞容积对有核红细胞富集效果的实验研究 | |
| 引用本文： | 程宁,刘芳,徐向红,张丽娜,白亚娜. 干预改变细胞容积对有核红细胞富集效果的实验研究[J]. 实用妇产科杂志, 2010, 26(4) |
| 作者姓名： | 程宁 刘芳 徐向红 张丽娜 白亚娜 |
| 作者单位： | 1. 兰州大学生殖健康与出生缺陷研究中心,甘肃,兰州,730000;兰州大学甘肃省新药临床前研究重点实验室,甘肃,兰州,730000 2. 兰州大学生殖健康与出生缺陷研究中心,甘肃,兰州,730000 3. 兰州大学公共卫生学院流行病学与卫生统计学研究所,甘肃,兰州,730000 |
| 基金项目： | 甘肃省"医药产学研"基金资助项目 |
| 摘 要： | 目的:通过观察干预脐血细胞K+/Cl-离子转运体(KCC)对细胞调节性容量减少(RVD)的生物学改变,为探讨富集胎儿有核红细胞(NRBC)方法提供线索.方法:流式细胞仪计数观察尿素干预脐血KCC转运体后不同密度介质下离心富集NRBC的效果.结果:在高渗400mmol/L尿素干预10分钟条件下,红细胞体积缩小最大(P<0.01).干预脐血后1.065 g/ml密度介质离心下层红细胞白膜层(未干预下层)NRBC富集量由12.20%升为69.90%,富集增长差异有统计学意义(u=7.96,P<0.05),单个核上层(未干预上层)NRBC由7.15%降为2.00%,减少量差异有统计学意义(u=5.47,P<0.05).结论:尿素干预脐血可以增加有核红细胞富集率,较传统富集法的富集量更高. |
| 关 键 词： | 有核红细胞 脐血 尿素 调节性容量减少 流式细胞术 |
The Study of the Nuclear Red Blood Cells Enrichment by Interfering Change of Cell Volume |
|
| CHENG Ning,,LIU Fang,XU Xianghong,et al. The Study of the Nuclear Red Blood Cells Enrichment by Interfering Change of Cell Volume[J]. Journal of Practical Obstetrics and Gynecology, 2010, 26(4) | |
| Authors: | CHENG Ning LIU Fang XU Xianghong et al |
| Affiliation: | CHENG Ning1,2,LIU Fang1,XU Xianghong1,et al(1.Center of Reproductive Health , Birth Defects,Lanzhou University,Lanzhou Gansu 730000,China,2.Key Laboratory of New Drug Preclinical Study in Gansu Province,China) |
| Abstract: | Objective:To observe the biological changes of cell regulatory volume reduction(RVD)by intervention of K+Cl-(KCC)in transporter in umbilical cord blood cells,in order to provide clues for the enrichment of fetal nucleated red blood cells(NRBC).Methods:The centrifugal effect of NRBC enrichment in different density media were counted by flow cytometry under urea intervention cord blood KCC transporter.Results:The volume of red blood cell decreased maximum under 400 mmolL hypertonic condition and interfering w... |
| Keywords: | Nucleated rsd blood cell Umbilical cord blood Urea Regulatory volume decrease Flow cytometry |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |