| Pinl抑制剂Juglone对食管癌EC1细胞增殖的抑制作用 |
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| 引用本文： | 李玲,陈平,连鸿凯,赵纪敏,马俊芬,董子明.Pinl抑制剂Juglone对食管癌EC1细胞增殖的抑制作用[J].世界华人消化杂志,2010,18(11). |
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| 作者姓名： | 李玲 陈平 连鸿凯 赵纪敏 马俊芬 董子明 |
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| 作者单位： | 1. 郑州大学基础医学院病理生理教研室,河南省郑州市,450001
2. 郑州市骨科医院,河南省郑州市,450001 |
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| 摘 要： | 目的:测定Pin1抑制剂(Juglone)对食管癌细胞EC1生长增殖的影响,探讨Juglone的抗肿瘤作用.方法:体外培养人食管癌细胞系EC1,用MTT试验观察细胞生长增殖状况,流式细胞仪检测细胞周期以及细胞凋亡.结果:MTT试验表明,Juglone对EC1细胞生长有明显的抑制作用,且抑制作用随作用浓度和作用时间增加而增强.流式细胞仪检测表明,加入Juglone培养48 h后,EC1细胞出现G2期阻滞.Juglone药物(10、20、30 μmol/L)培养48 h后,EC1细胞的凋亡率明显增加,与对照组相比有统计学意义(9.06%,32.88%,53.18% vs 8.77%.均P<0.05).结论:Pin1抑制剂Juglone可以通过抑制Pin1表达从而抑制食管癌细胞的增殖,Pin1抑制剂有望成为新型的抗肿瘤治疗靶点.
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| 关 键 词： | 肽酰脯氨酰顺反异构酶 细胞凋亡 细胞周期 |
Juglone inhibits the proliferation of human esophageal carcinoma EC1 cells |
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| Ling Li,Ping Chen,Hong-Kai Lian,Ji-Min Zhao,Jun-Fen Ma,Zi-Ming Dong.Juglone inhibits the proliferation of human esophageal carcinoma EC1 cells[J].World Chinese Journal of Digestology,2010,18(11). |
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| Authors: | Ling Li Ping Chen Hong-Kai Lian Ji-Min Zhao Jun-Fen Ma Zi-Ming Dong |
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| Institution: | Ling Li,Ping Chen,Ji-Min Zhao,Jun-Fen Ma,Zi-Ming Dong,Department of Pathophysiology,Basic Medical College,University of Zhengzhou,Zhengzhou 450001,Henan Province,China Hong-Kai Lian,Orthopedic Hospital of Zhengzhou,China |
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| Abstract: | AIM: To investigate whether juglone, an inhibitor of Pin1, inhibits the proliferation of human esophageal carcinoma cells (EC1) in vitro. METHODS: Cultured EC1 cells were incubated with different concentrations of juglone for different durations. The proliferation of EC1 cells was detected by methyl thiazolyl tetrazolium (MTT) assay. The cell cycle distribution and cell apoptosis were measured by flow cytometry (FCM). RESULTS: MTT results showed that juglone treatment decreased the proliferation of EC1 cell... |
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| Keywords: | Juglone |
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