心肌联合注射纤维蛋白封闭剂在细胞移植中应用的研究

目的:探讨心肌注射骨髓间充质干细胞(mesenchymal stemcells,MSCs)联合应用纤维蛋白封闭剂(FibrinSealant,FS),对细胞滞留量的影响。方法:(1)提取、分离、培养、扩增小型猪骨髓MSCs备用;(2)小型猪随机分为2组,成功建立急性心肌梗塞模型,然后分为单纯MSCs移植组和MSCs联合纤维蛋白封闭剂移植组,对比移植6周后细胞的滞留量及新生血管的密度,超声学评价心脏功能。结果:6周后MSCs FS组荧光标记的MSCs数量、新生血管密度均较MSCs组显著增多(P<0.01),MSCs FS组左室短轴缩短率(FS%)、心肌射血分数(EF%)较MSCs组显著提高(P<0.05,<0.01)。结论:经心外膜心肌注射MSCs联合纤维蛋白封闭剂较单纯心肌注射MSCs能够明显提高移植细胞的滞留存活数量,增加新生血管密度,改善心肌收缩功能。

Experimental studies of intramyocardial injecting bone marrow mesenchymal stem cells combined with fibrin sealant in cells transplantation

Objective:To investigate the effects of intramyocardial injecting bone marrow mesenchymal stem cells combined with fibrin sealant in cells transplantation.Methods:(1)Prepared for isolation,purification,culture,expansion of bone marrow MSCs.(2)Mini-swine were randomly assigned to establish models of acute myocardial infarction and then were performed by the subsequent procedure:intramyocardial MSCs injection alone(Group MSCs)and intramyocardial MSCs injection combined with FS(Group MSCs FS).Six weeks postoperatively,quantitative analysis of fluorescent-labeled cells was performed by Image Pro Plus 4.5 software.Likewisely,neovascularization by histochemically staining was analyzed as well.Cardiac function was assessed by echocardiorgaphy.Results:Six weeks postoperatively,both the retention number of positively labeled cells and new vessels density in Group MSCs FS showed significant increase compared with those in Group MSCs(P<0.01).Myocardial function of EF% and FS% in Group MSCs FS markedly improved compared with that in Group MSCs(P<0.05,<0.01).Conclusion:Intramyocardial injecting bone marrow MCSs combined with fibrin sealant can increase the retention of transplanted cells,promote neovascularization formation and enhance myocardial systolic function.