不同粗糙表面的纯钛种植体表面的体外细胞培养评价

目的：本文通过对不同粗糙度表面纯钛种植体（光滑、机械表面、大粗糙表面、2种不同的微粗糙表面）的体外细胞培养评价,寻找具有较好的细胞附着的表面状态。方法：对5种不同粗糙度的钛种植体表面进行2BS成纤维细胞培养,通过光镜和电子显微镜对材料表面的成纤维细胞的数量和形态、细胞的粘附状态和细胞与种植体的关系进行了观察和分析。结果：粗糙表面的钛种植体对成纤维细胞的＂诱导能力＂更强,表现为细胞迁徙、附着于二者的细胞数目比光滑表面的钛板为多,细胞桥形成更早。结论：钛种植体表面微粗糙化提高钛材的生物相容性。

Testing by cell culture in vitro of pure titanium implant with different surface microroughness

Objective：The purpose of this test was to find out the better surface for 2BS cell sticking.Methods： In terms of the surface roughness,titanium samples are divided into 5 groups： smooth surface,machining surface,2 different microrough surfaces and macrorough surface.We cultivated 2BS cells on the each surface,and observed： （1）the amount and morphology of cell ;（2）cell sticking on the side of materials;（3）the relationship between cell and materials using light microscopy and canning electron microscopy.Quantitative analysis to the cells sticking on the side of the materials was performed.Results：The implant with rough surface was stronger ability to induce 2BS cell migrating、 sticking and forming of cell -bridge.Conclusion：Surface roughness of the titanium implant will improve the Biocompatibility of the implant.