| 慢性牙周炎龈下菌斑和唾液微生物群落分析 |
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| 引用本文： | 刘莹,吴明桃,陈慧,庞小燕,赵立平,唐子圣.慢性牙周炎龈下菌斑和唾液微生物群落分析[J].牙体牙髓牙周病学杂志,2011(5):254-258,275. |
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| 作者姓名： | 刘莹 吴明桃 陈慧 庞小燕 赵立平 唐子圣 |
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| 作者单位： | 上海交通大学医学院附属第九人民医院·牙体牙髓科上海市口腔医学重点实验室;复旦大学附属华山医院口腔科;上海交通大学生命科学技术学院微生物分子生态学与基因组学实验室; |
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| 基金项目： | 国家重点基础研究发展计划资助(2007CB936004); 上海交大校科研基金(2008XJ028),上海交大“医工(理)交叉研究基金”(YG2010MS61) |
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| 摘 要： | 目的:运用DGGE技术检测慢性牙周炎龈下菌斑和唾液微生物群落结构,分析其菌群之间的关系。方法:选取34名慢性牙周炎志愿者,分别采集龈下菌斑和唾液样本,采用PCR扩增细菌16Sr RNA V3区后,运用DGGE技术分析各组样本微生物群落结构。通过数字化软件Im age J将DGGE凝胶图谱转成数字信息,对所得矩阵进行主成分分析(Princ ipal ComponentAnalysis,PCA)、聚类分析(C luster Analysis)和偏最小二乘法(Partial Least Squares,PLS)分析。结果:DGGE图谱显示:龈下菌斑DGGE条带数目为11～28,,平均19;唾液DGGE条带数目为11～24,平均17;PCA结果显示:龈下菌斑与唾液比较有明显的差异;聚类分析显示:龈下菌斑、唾液形成5个明显聚类群,在同一聚类群中相似度高,在不同聚类群中相似度低,表示龈下菌斑与唾液菌群之间有显著差异;PLS结果显示:慢性牙周炎龈下菌斑与唾液菌群有显著差异。结论:DGGE是一种能直观显示微生物群落的指纹技术。通过该技术能检测牙周、唾液微生物群落的结构和组成,本结果显示,慢性牙周炎龈下菌斑与唾液微生物群落结构有明显差异。
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| 关 键 词： | 微生物群落 龈下菌斑 唾液 DGGE 慢性牙周炎 |
The microbiotal composition of subgingival plaque and saliva in chronic periodontitis |
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| LIU Ying,WU Ming-tao,CHEN Hui,PANG Xiao-yan,ZHAO Li-ping,TANG Zi-sheng.The microbiotal composition of subgingival plaque and saliva in chronic periodontitis[J].Chinese Journal of Conservative Dentistry,2011(5):254-258,275. |
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| Authors: | LIU Ying WU Ming-tao CHEN Hui PANG Xiao-yan ZHAO Li-ping TANG Zi-sheng |
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| Institution: | LIU Ying,WU Ming-tao,CHEN Hui,PANG Xiao-yan,ZHAO Li-ping,TANG Zi-sheng(Department of Endodontic,Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai Key Laboratory of Stomatology,Shanghai 200011,China) |
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| Abstract: | AIM:To detect and analyze the microbiotal composition and relationship of subgingival plaque and saliva by denaturing gradient gel electrophoresis(DGGE) in chronic periodontitis patients.METHODS:Thirty-four chronic periodontitis patients with no systematic disease were enrolled.Their subgingival plaque and saliva samples were collected to detect the microbiological profiles.V3 region of the 16S rRNA gene of all bacteria was amplified by PCR and separated by DGGE.The band patterns were digitized by Image J,a... |
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| Keywords: | microbial community subgingival plaque saliva DGGE chronic periodontitis |
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