In vitro and in vivo MMP gene expression localisation by In Situ -RT-PCR in cell culture and paraffin embedded human breast cancer cell line xenografts |
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| Authors: | Larisa M Haupt Erik W Thompson Ann EO Trezise Rachel E Irving Michael G Irving and Lyn R Griffiths |
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| Institution: | (1) Genomics Research Centre, Griffith University Gold Coast, School of Medical Science, Griffith University, 4217 Queensland, Australia;(2) IMCB, Biopolis, Singapore;(3) Department of Surgery, VBCRC Invasion and Metastasis Unit, St. Vincent's Institute of Medical Research and University of Melbourne, 3065 Victoria, Melbourne, Australia;(4) School of Biomedical Sciences, University of Queensland, St Lucia, 4072 Queensland, Australia;(5) Institute of Health Sciences, Bond University, 4229 Queensland, Australia |
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| Abstract: | Background Members of the matrix metalloproteinase (MMP) family of proteases are required for the degradation of the basement membrane
and extracellular matrix in both normal and pathological conditions. In vitro, MT1-MMP (MMP-14, membrane type-1-MMP) expression is higher in more invasive human breast cancer (HBC) cell lines, whilst
in vivo its expression has been associated with the stroma surrounding breast tumours. MMP-1 (interstitial collagenase) has been
associated with MDA-MB-231 invasion in vitro, while MMP-3 (stromelysin-1) has been localised around invasive cells of breast tumours in vivo. As MMPs are not stored intracellularly, the ability to localise their expression to their cells of origin is difficult. |
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