| Ag85A DNA疫苗对肠上皮内淋巴细胞FasL mRNA表达的影响 |
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| 引用本文： | 王庆辉,吕昌龙,Azuma KALU,吕超,方芳,李季.Ag85A DNA疫苗对肠上皮内淋巴细胞FasL mRNA表达的影响[J].中国医科大学学报,2006,35(3):225-226. |
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| 作者姓名： | 王庆辉 吕昌龙 Azuma KALU 吕超 方芳 李季 |
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| 作者单位： | 1. 中国医科大学基础医学院免疫学教研室,辽宁,沈阳,110001
2. 沈阳医学院临床医疗系 |
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| 摘 要： | 目的：分析口服Ag85A DNA疫苗对小鼠肠上皮内淋巴细胞（iIEL）FasLmRNA表达的影响.方法：减毒伤寒杆菌运载的Ag85A DNA疫苗经口免疫小鼠,检测iIEL和脾淋巴细胞FasL mRNA的表达.结果：末次免疫后8 d,Ag85A DNA质粒组小鼠iIEL FasL mRNA平均表达水平高于正常对照组和空质粒对照组;空质粒对照组和正常对照组的iIEL FasL mRNA平均表达水平以及3组脾淋巴细胞FasL mRNA的表达水平均未见显著性差异.结论：口服Ag85A DNA疫苗可以上调小鼠iIEL FasLmRNA的表达,而对脾淋巴细胞未见有意义的影响.
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| 关 键 词： | Ag85A DNA疫苗 肠上皮内淋巴细胞 黏膜免疫 |
| 文章编号： | 0258-4646(2006)03-0225-02 |
| 修稿时间： | 2005年6月10日 |
Effect of Ag85A DNA vaccine on FasL mRNA expression in intestinal intraepithelial lymphocytes |
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| WANG Qing-hui,L Chang-long,Azuma KALU,L Chao,FANG Fang,LI Ji.Effect of Ag85A DNA vaccine on FasL mRNA expression in intestinal intraepithelial lymphocytes[J].Journal of China Medical University,2006,35(3):225-226. |
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| Authors: | WANG Qing-hui L Chang-long Azuma KALU L Chao FANG Fang LI Ji |
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| Institution: | Azuma KALU,WANG Qing-hui,L(U) Chang-long,Azuma KALU,L(U) Chao,FANG Fang,LI Ji |
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| Abstract: | Objective: To analyze the effect of oral Ag85A DNA vaccine on FasL mRNA expression in the intestinal intraepithelial lymphocytes (iIEL) of mice.Methods: The expressions of FasL mRNA in both iIEL and spleen lymphocytes were determined after oral immunization to mice using Ag85A DNA vaccine carried by attenuated Salmonella typhimurium.Results: On day 8 after last immunization, the expression of FasL mRNA in iIEL in Ag85A plasmid group was higher than that in both normal control group and empty plasmid control group. There was no significant difference in FasL mRNA expression in iIEL between normal control group and empty plasmid control group, and there was no significant difference in Fasl mRNA expression in spleen lymphocytes among these 3 groups. Conclusion: Oral Ag85A DNA vaccine could upregulate the expression of FasL mRNA in iIEL. |
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| Keywords: | Ag85A DNA vaccine intestinal intraepithelial lymphocytes mucosal immunity |
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