人参总皂甙对乳酸盐腹膜透析液致人腹膜间皮细胞损伤的保护作用

目的 :观察人参总皂甙 (GS)对乳酸盐腹膜透析液 (L -PDS)在体外所致的人类腹膜间皮细胞 (HPMC)活力和增殖抑制的影响。方法 :采用胰蛋白酶消化法从人腹膜组织中分离间皮细胞 ,建立稳定的体外培养模型 ;用乳酸脱氢酶 (LDH)的释放和四甲基偶氮唑盐比色法 (MTT法 )评估细胞的活性及增殖能力。结果 :2 .5 %和 4.2 5 %葡萄糖L-PDS组与对照组相比HPMC的LDH释放增多 ,且两浓度差异无显著性 ;随L -PDS暴露时间的延长 ,HPMC的存活率逐渐降低 ;GS可以减少LDH的释放 ,提高细胞存活率 (P <0 .0 1) ,并能促进HPMC的增殖 (P <0 .0 5 )。结论 :GS对L-PDS体外所致HPMC活力和细胞增殖的抑制具有一定的保护作用。

Protective effect of ginsenosides on human peritoneal mesothelial cell against lactate-based peritoneal dialysis solutions-induced injury in vitro

Objective The aim of this research was to observe the influence of ginsenosides (GS) on viability and proliferation of human peritoneal mesothelial cell (HPMC) which were inhibited by L-PDS in vitro. Method Mesothelial cells were isolated from human omental specimens by trypsin disaggregation and established a stable HPMC culture model. The viability and capacity of proliferation of HPMC were assessed by lactate dehydrognase (LDH) release and tetrazolium salt colorimetry assay (MTT assay). Results Compared with the control group, the release of LDH in L-PDS groups (2.5%, 4.25% glucose) significantly increased. But there was no difference between the two glucose concentrations. As HPMC pre-exposed to L-PDS prolonged, the viability of cells decreased gradually. GS decreased the release of LDH and increased the cells viability (P<0.01), as well as enhanced the proliferation of HPMC. Conclusion The results suggest that GS has some protective effects on cell viability and proliferation of HPMC inhibited by L-PDS in vitro