脑胶质瘤组织PTEN mRNA表达及其基因突变SSCP分析

目的：探讨PTEN基因表达水平及其基因突变与脑胶质瘤的发生及恶性进展的关联性。方法：选取脑胶质瘤组织15例，以4例非胶质瘤脑组织为对照，采用逆转录聚合酶链反应(RT-PCR)法检测脑胶质瘤组织PTEN mRNA表达水平，采用单链构象多态性分析法（SSCP analysis）快速筛查脑胶质瘤组织PTEN基因突变状况。结果：RT-PCR结果，脑胶质瘤组织PTEN mRNA表达水平(0.063±0.006)较对照组(0.126±0.015)明显降低（P<0.05）。经单链构象多态性分析，脑胶质瘤组织PTEN基因外显子8、9未检测到突变，但其中1例PTEN基因外显子5的聚丙烯酰胺凝胶电泳结果显示电泳带飘移，考虑可能为杂合子突变。结论：PTEN基因低表达及基因突变可能对胶质瘤发生、发展有促进作用。

Expression of PTEN mRNA in human brain glioma tissue and its SSCP analysis of gene mutation

Objective To study the relationships between the expression level of PTEN gene, gene mutation and occurrence and development of glioma.Methods Fifteen glioma tissues were selected,and the normal 4 non-glioma tissues were used as control.The expression level of PTEN mRNA was measured by RT-PCR method,and the mutation of PTEN gene was screened by SSCP analysis in various histopathological grades of glioma.Results Compared with control group(0.126±0.015),the level of PTEN mRNA in glioma tissues(0.063±0.006) was decreased(P<0.05).SSCP analysis showed that there was not mutation of the bands of exon 8 and 9 of PTEN gene .The band shift of exon 5 of PTEN gene of one specimen was found on ployacrylamide gel electrophoresis.Conclusion The low expression and mutation of PTEN gene probably promote the occurrence and development of glioma.