脑胶质瘤中骨桥蛋白表达初步研究

目的研究胶质瘤中骨桥蛋白（OPN）mRNA表达及其临床意义。方法应用半定量RT—PCR法检测OPNmRNA在60例胶质瘤及相应正常脑组织中的表达及相对含量，分析其与胶质瘤临床病理特征和预后之间的关系。结果本组OPNmRNA的阳性表达率为73．3％（44／60），正常脑组织OPNmRNA表达均为阴性，OPNmRNA的相对表达量与肿瘤临床病理分级有关；所有患者平均随访12个月，OPN表达阳性组44例中复发36例，表达阴性组16例中仅复发2例（P〈0．05），此外表达阳性组中死亡23例，表达阴性组仅2例死亡（P〈0．05）。结论胶质瘤中骨桥蛋白mRNA表达明显增高，并与肿瘤临床病理分级密切相关，骨桥蛋白可作为一临床评价胶质瘤患者预后的良好指标。

Preliminary Study on Expression of Osteopontin in Human Gliomas

Objective To investigate the expression of osteopontin(OPN) mRNA and its correlation with clinicopathologic features of glioma. Methods The expression of OPN mRNA was detected by semi-quantitive RT-PCR in 60 cases of gliomas and their correspondent normal tissues.The relationship between the relative content of OPN mRNA and clinicopathologic features of glioma was also analyzed. Results The positive rate of OPN mRNA expression was 73.3%(44/60) in our group.The OPN mRNA expression in normal brain tissue was all negative.Furthermore,the OPN mRNA expression was associated with the pathological grade of glioma.All patients were followed up for an average of 12 months.We did observed that the OPN mRNA expression positive group(44 cases) had recurrence in 36 patients and the negative group(16 cases) had only 2 case with recurrence (P<0.05);23 patients died in OPN mrna expression positive group but only two patients died in OPN staining negative group(P<0.05).Conclusions OPN mRNA is over-expressed in glioma and may play an important role in the tumor progression,which also may be taken as a leading candidate for evaluation the prognosis of glioma.