成人骨髓间充质干细胞生长特性及bFGF对其增殖的影响

目的：研究成人骨髓间充质干细胞（hMSC）生长特性、hMSC表面标记及bFGF对hMSC增殖的影响。 方法：绘制细胞生长曲线；流式细胞仪检测细胞表面标记；MTT法检测bFGF对hMSC增殖的影响。 结果：原代培养细胞生长潜伏期5~9 d，对数增殖期11~16 d，生长平台期18~20 d；传代培养细胞生长潜伏期12~24 h，对数增殖期为7~10 d，11~13 d进入细胞生长平台期。hMSC均一表达CD44，不表达CD45。bFGF在较低浓度（10 μg•L^-1）时即有显著的促进hMSC增殖的作用（P<0.05）， bFGF在25~100 μg•L^-1对hMSC的ALP活性有较显著的抑制作用。 结论：hMSC在体外可迅速扩增；bFGF对hMSC的增殖作用与浓度成正性剂量-效应关系。

Biological features of hMSC and effects of bFGF on its proliferation

Objective To study the growth pattern and surface makers of adult human mesenchymal stem cells (hMSC) and the influences of bFGF on the proliferation of hMSC.Methods The growth curves of the hMSC were drawn; FACS was used to detect surface antigenic markers. Cell growth pattern and its response to bFGF were evaluated by MTT test. Results The latent phase of the primary culture cells was 5-9 days, followed by 11-16 days of logarithmical proliferation; the plateau began at day 18-20. The subcultures showed 12-24 hours of latent phase, followed by 7-10 days of logarithmical proliferation, then entered the plateau (11-13 days). The hMSC expressed positive CD44 unanimously, but don′t expressed CD45. Low concentration of bFGF (10 μg·L -1) could stimulate the proliferation of hMSC significantly (P<0.05); while 25-100 μg·L -1 of bFGF could inhibit the ALP activity. Conclusion hMSC can be expanded in vitro rapidly. The effect of bFGF on hMSC proliferation is in positive dose-effect manner.