新疆草棉花总黄酮系统药效学研究

目的: 研究草棉花瓣提取物总黄酮(FGF)的毒性、抗炎免疫等方面的药效学,为研究和开发新疆的地方药用植物奠定基础。方法: 采用二甲苯致小鼠耳廓肿胀,角叉菜胶致小白鼠足跖肿胀,棉球致大鼠肉芽肿,测定炎性组织中PGE2 和TXA2、血清溶菌酶含量、小鼠脾巨噬细胞白介素1的活性,观察FGF的抗炎作用。测定小鼠免疫器官相对重量、巨噬细胞吞噬中性红能力、血清溶血素含量,采用四甲基偶氮唑盐比色法(MTT法)测定淋巴细胞增殖反应,邻苯三酚自氧化法测SOD活性,DTNB直接法测GSH PX活性,TBA显示色法测MDA含量,观察FGF对免疫功能的影响。结果: FGF(300、600 mg/kg×7 d)对各种急、慢性炎症均有明显的抑制作用(P <0.01),FGF不同剂量组对绵羊红细胞攻击小鼠可增加脾腺指数,降低胸腺指数,呈剂量依赖性;FGF(600 mg/kg×7 d)能增加腹腔巨噬细胞的吞噬能力(P<0.01);FGF(300、600 mg/kg×7 d)可使半数溶血值(HC50)明显升高(P<0.01); FGF(300 mg/kg×l5 d)可增加IL 1活性,促进脾淋巴细胞增殖(P<0.05);FGF(150 mg/kg×l5 d)可增加心肌GSH PX、肝SOD活性,降低肾MDA含量(P

A systematic research on the pharmacodynamics of Flos Gossipium Flavonoid in Xinjiang

Objective: To study the toxicity, anti-inflammatory and immune enhancing properties of Flos Gossipium Flavonoid (FGF) in Xinjiang and contribute to full exploitation of medicinal herbs in Xinjiang. Methods: Acute inflammation models were established by using xylene in mice ear, carregenin in mice paw and cotton pellets in rats, respectively. FGF's anti-inflammatory effects were observed by assessing the changes in the levels of the PGE\-2, TXA\-2 in the inflammatory tissues, IL-1 from spleen macrophages, relative weights of mice lymphoid organs and production of hymolysin in mice. To observe the effects of FGF on the immune functions, superoxide dismutase (SOD) activity was assessed by progalllic acid autooxidation method, GSH-PX activity was determined by the 5,5-dithio-bis (2-nitrobenzoic acid) (DTNB) method and malondialdehyde (MDA) content was assessed by thiobarbituric acid (TBA) method, respectively. Results: Maximal tolerance dose for FGF was 10 g/kg in mice; Anti-inflammatory effects of FGF (300, 600 mg/kg for 7 days) found in both acute and chronic inflammation models (P<0.01). FGF at different doses showed an increase in the spleen index and a decrease in the thymus index in sheep erythrocyte induced models in mice and showed an apparent dose dependency. FGF (600 mg/kg for 7 days) elevated the phagocytosis of mouse peritoneal macrophages (P<0.01); FGF (300, 600 mg/kg for 7 days) markedly increased the hemolysis (HC 50) (P<0.01); FGF (300 mg/kg for 15 days) enhanced the activity of IL-1, the proliferation of spleen lymphoctes (P<0.05); FGF (150 mg/kg for 15 days) increased the SOD activity in the liver and GSH-PX in cardiomyocytes while decreased MDA content in the kidney (P<0.05); FGF increased the GSH-PX levels in the cardiomyocytes and the kidney but decreased the SOD activity and MDA content (P<0.05); FGF increased the SOD activity in the kidney and lowered MDA content in the liver (P<0.05) and markedly decreased the GSH-PX activity in the cardiomyocytes and MDA content in the kidney (P<0.05).Conclusion: FGF had a relatively low toxicity and possesses anti-inflammatory and immune enhancing effects.