参附注射液对休克复苏时肠黏膜保护作用机制的实验研究

目的 探讨参附注射液（SF）对休克复苏期间肠黏膜损伤的保护作用机制。方法 通过兔失血性休克复苏模型，21只家兔随机分为参附注射液治疗组（A组）、单纯复苏组（B组）和假手术组（C组）；A组于复苏同时首剂静注参附注射液2．1ml/kg，随后静滴5ml/kg，直到观察结束。分别于实验前（S0）、休克1h（S1）及复苏1h(R1)、3h(R3）观察乙状结肠黏膜内pH（pHi)、肠粘膜NO、MDA及钙（Ca^ )含量。结果 A组R1和R3时肠pHi明显高于B组相应值（P＜0．05），R3时肠黏膜NO、MDA及Ca^ 含量低于B组（均P＜0．05）；B组复苏期间场pHi维持在S1时的低水平状态，肠黏膜NO、MDA及Ca^ 含量均明显高于A组和C组（P＜0．05）。结论 SF对失血性休克复苏期间肠黏膜保护作用的机制可能为增加肠黏膜灌注及氧合、抑制NO的产生及抗炎效应、清除氧自由基及减轻钙超载。

Protective effect of shenfu injection on intestinal mucosa during hemorrhagic shock-reperfusion in rabbits

Objective To investigate the mechanism of protective effect of shenfu(SF) on imtestinal mucosa during hemorrhagic shock-reperfusion in rabbits.Methods Using the rabbit model of hemorrhagic shock-reperfusion,twenty-one rabbits were divided randomly into 3 groups:SF groups(A),Shock-reperfusion Group(B)and control group(C).Intestinal intramucosal pH(pHi)of sigmoid colon,nitric oxide(NO),malondialdehyde(MDA)and Ca contents of intestinal mucosa were measured before shock(S 0 ),1 hour after shock(S 1 ),1 hour(R 1 )and 3 hour(R 3 )of reperfusion.Results pHi in group A were significantly higher than those in group B during reperfusion.NO,MDA and Ca contents of intestinal mucosa were significantly lower at R 3 than those in group B respectively ( P <0.05).Compared with those in group C,NO and MDA contents of intestinal mucosa remained unchanged,but Ca contents of intestinal mucosa increased significantly in group A at R 3 .Conclusions SF can protect intestinal mucosa from ischemia-reperfusion injury during hemorrhagic shock through improving perfusion and oxygenation,inhibiting the production of NO and anti-inflammation action,reducing oxide free radical and calcium overload.