种植窗期人子宫内膜组织SDF-1α/CXCR4的表达

目的 研究不同卵巢反应性的种植窗期子宫内膜组织中基质细胞衍生因子-1α(SDF-1α)及其受体CXCR4的表达.方法 收集种植窗期子宫内膜组织(n=17),根据卵巢反应性分为卵巢高反应组(n=6)、卵巢中等反应组(n=6)和正常对照组(n=5).应用RT-PCR、Real-time PCR、免疫组织化学染色和Western blotting方法 ,检测并比较各组种植窗期子宫内膜组织SDF-1α/CXCR4 mRNA和蛋白表达.结果 免疫组织化学染色显示,CXCR4在种植窗期子宫内膜组织中高表达;SDF-1α在内膜腺体和腔上皮细胞中表达,而在问质细胞中不表达.各组子宫内膜组织SDF-1α/CXCR4 mRNA和蛋白表达比较,差异均无统计学意义(P>0.05).结论 高甾体激素对种植窗期子宫内膜趋化因子SDF-1α及其受体CXCR4的表达无明显影响.提示SDF-1α/CXCR4可能不参与甾体激素对种植窗期CD56bright CDl6-NK细胞募集的调控.

Expression of SDF-1α/CXCR4 in human endometria of peri-implantation phase

Objective To investigate the expression of stromal cell-derived factor-1α (SDF-1α) and CXCR4 under different ovarian responses in human endometria of peri-implantation phase. Methods Endometria of peri-implantation phase (n=17) were collected, and were divided into moderate ovarian response group (n=6), high ovarian response group (n=6) and normal control group (n=5). The expression of SDF-1α/CXCR4 mRNA and protein in endometria of peri-implantation phase was detected and analysed by RT-PCR, Real-time PCR, immunohistochemistry and Western blotting. Results It was revealed by immunohistochemistry that CXCR4 was highly expressed in endometrial tissues, whereas SDF-1α was confined to glandular and celomic epithelial cells instead of stromal cells. There was no significant difference in the expression of SDF-1α/CXCR4 mRNA and protein in human endometria among each group (P>0.05). Conclusion High levels of steroid hormone do not affect expression of SDF-1α/CXCR4 in human endometria of peri-implantation phase, indicating that SDF-1α/CXCR4 may not participate in the recruitment of CD56brightCD16-NK cells to endometria by steroid hormone.