| 神经元兴奋性毒性对Munc18-1蛋白在大鼠海马神经元和原代培养神经元胞核内分布的影响(英文) |
| |
| 引用本文： | 张彦平,万萍,王洪权,赵红,许玉霞,杨茹,朱粹青. 神经元兴奋性毒性对Munc18-1蛋白在大鼠海马神经元和原代培养神经元胞核内分布的影响(英文)[J]. 中国神经科学杂志, 2011, 0(3) |
| |
| 作者姓名： | 张彦平 万萍 王洪权 赵红 许玉霞 杨茹 朱粹青 |
| |
| 作者单位： | 复旦大学上海医学院医学神经生物学国家重点实验室;复旦大学脑科学院; |
| |
| 基金项目： | supported by grants from the National Natural Science Foundation of China (No. 81071017, 30470536, 90919004) |
| |
| 摘 要： | 目的 Munc18-1在中枢神经系统递质释放过程中具有重要作用,控制着突触囊泡释放步骤的每一个环节。Munc18-1功能异常与癫痫发病相关。本文主要探讨癫痫是否会引起神经元胞核内Munc18-1定位的改变。方法通过海马内注射海人藻酸建立Sprague-Dawley(SD)大鼠癫痫模型,腹腔注射海人藻酸建立昆明小鼠癫痫模型。分离胎龄18天SD大鼠海马神经元,用Neurobasal培养基培养7天后,用谷氨酸处理3h。用蔗糖密度梯度离心法分离神经元和神经胶质细胞的细胞核组份,通过甲酚紫染色对上述富含细胞核的组份进行形态学鉴别。用免疫组化和免疫电镜分析法确定Munc18-1的细胞核定位。免疫印迹法检测不同细胞组分的Munc18-1蛋白的表达水平。结果免疫组化、免疫电镜以及对神经元细胞核组份的免疫印迹证实了Munc18-1在海马神经元细胞核的分布定位。在海人藻酸诱导癫痫的动物海马内,免疫组化染色显示Munc18-1在海马CA区锥体细胞层、齿状回颗粒细胞层和门区的多型细胞表达减少。同时,免疫印迹分析表明,Munc18-1在海马神经元胞核中的表达水平明显下降。免疫印迹检测显示,原代培养神经元经50μmol/L谷氨酸处理3h...
|
| 关 键 词： | Munc18-1 细胞核 海人藻酸 谷氨酸 海马 原代培养神经元 |
Effect of neuronal excitotoxicity on Munc18-1 distribution in nuclei of rat hippocampal neuron and primary cultured neuron |
| |
| Yan-Ping ZHANG,Ping WAN,Hong-Quan WANG,Hong ZHAO,Yu-Xia XU,Ru YANG,Cui-Qing ZHU, State Key Laboratory of Medical Neurobiology. Effect of neuronal excitotoxicity on Munc18-1 distribution in nuclei of rat hippocampal neuron and primary cultured neuron[J]. Neuroscience Bulletin, 2011, 0(3) |
| |
| Authors: | Yan-Ping ZHANG Ping WAN Hong-Quan WANG Hong ZHAO Yu-Xia XU Ru YANG Cui-Qing ZHU State Key Laboratory of Medical Neurobiology |
| |
| Affiliation: | Yan-Ping ZHANG1,Ping WAN1,Hong-Quan WANG1,Hong ZHAO1,Yu-Xia XU1,Ru YANG1,Cui-Qing ZHU1,2 1State Key Laboratory of Medical Neurobiology and 2Institutes of Brain Science,Fudan University,Shanghai 200032,China |
| |
| Abstract: | Objective Munc18-1 has an important role in neurotransmitter release, and controls every step in the exocytotic pathway in the central nervous system. In the present study, whether epileptic seizure causes a change of Munc18 localization in neuronal nuclei was analyzed. Methods Epilepsy models were established by injection of kainic acid (KA) solution into hippocampus of Sprague-Dawley (SD) rats or intraperitoneal injection of KA in Kunming mice. The hippocampal neurons were prepared from embryonic day 18 S... |
| |
| Keywords: | Munc18-1 nucleus kainic acid glutamate hippocampus primary cultured neurons |
| 本文献已被 CNKI 等数据库收录！ |
|
