| 逆转录病毒介导人肿瘤坏死因子基因治疗胶质瘤的实验研究 |
| |
| 引用本文： | 王晨,沈兆忠.逆转录病毒介导人肿瘤坏死因子基因治疗胶质瘤的实验研究[J].中国临床神经科学,1998,6(3):149-153. |
| |
| 作者姓名： | 王晨 沈兆忠 |
| |
| 作者单位： | 上海华山医院神经外科!200040(王晨，周良辅)，上海肿瘤医院分子生物实验室!200032(沈兆忠，罗建民) |
| |
| 摘 要： | 运用小鼠白血病病毒MoMLV为载体,将TNFa基因转导致人胶质瘤细胞SHG-44,14dG418筛选出细胞克隆.检测TNFa的表达.观察细胞生长.结果:转基因细胞培养上清液中有高浓度TNFa表达分别为(5 198.7±3 757.4)pg/ml和(3 217.4±1 180.6)pg/ml(P<0.05),生物活性达320.0U/ml.“RT-PCR”检测转基因细胞有特异mRNA表达,转基因细胞生长减慢.转染TNFa基因可诱导胶质瘤细胞的生长抑制.
|
| 关 键 词： | 胶质瘤 TNFα 基因治疗 |
Fundamental Study in Gene Therapy of Human Glioma Cells Transduced by Human Tumor Necrosis Factor Gene |
| |
| Wang Chen,Zhou Liang-Fu,Shen Zhao-Zhogn,Luo Jian-Min.Fundamental Study in Gene Therapy of Human Glioma Cells Transduced by Human Tumor Necrosis Factor Gene[J].Chinese Journal of Clinical Neurosciences,1998,6(3):149-153. |
| |
| Authors: | Wang Chen Zhou Liang-Fu Shen Zhao-Zhogn Luo Jian-Min |
| |
| Institution: | Wang Chen,Zhou Liang-Fu,Shen Zhao-Zhogn,Luo Jian-Min Department of Neurosurgery,Hua Shan Hospital,Shanghai Medical University,Shanghai 200040"Department of Molecular Biology,Shanghai Tumor Hospital,Shanghai 200042 |
| |
| Abstract: | |
| |
| Keywords: | glioma tumor necrosis factor gene therapy |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
