协调性异种肝移植免疫病理学的实验研究

观察协调性异种肝移植免疫病理变化,探讨其免疫排斥反应机制。方法：建立仓鼠到大鼠原位肝移植模,运用组织学方法检测异种／肝移植受体脾脏、淋巴结的组织学的改变;采用免疫组织化学的方法检测移植肝大鼠抗体的沉着、淋巴细胞的浸润及穿孔素、Ｆａｓ－Ｌ的表达;原位末端标记方法,检测植肝细胞凋亡。

The immunopathology in concordant liver xenograft

Objectives: To investigate the immunopathologic change in the rejection of liver xenograft. Methods: After orthotopic liver transplantation model was established, the histologic change of spleen,lymphocyte node and the graft was observed histologically; the expression of perforin and Fas L and the deposits of antibody was detected by immunohistochemistry; the apoptosis in grafts was examined by histology and TUNEL. Results: In the xenogenic rejection,the spleen and lymphocyte node proliferate rapidly and become larger. In the graft, the antibody deposits, the T lymphocyte infiltration,the expression of Fas L and perforin could be detected, and increasing as time passes, the incidence of hepatocyte apoptosis increased markedly,too. Conclusion: In the xenogenic rejection,the antibody and T cell mediated cytotoxicity plays an important role, T cell make the target cell necrotic or apoptotic by the pathway of Fas L and perforin.