大鼠慢性酒精性脑损害及额区MDA、MT含量变化的研究

目的观察慢性酒精性脑损害大鼠额区脑组织丙二醛、金属硫蛋白含量变化情况。方法通过逐步增加浓度自由饮方法建立慢性酒精性脑损害大鼠动物模型。观察大鼠额区脑组织的形态学改变,测定脑组织中MOD、MT水平。结果酒精组额区脑组织有神经细胞数目缺失及细胞变性和损伤,酒精组MDA含量显著升高,MT含量显著降低,与对照组比较差异有统计学意义(P<0.01)。结论长期摄入大量酒精可使脑组织氧自由基代谢失衡,MDA含量升高,MT含量降低。

Study on the brain damage and MDA,MT changes in frontal area of chronic alcoholic rats

Objective To observe the levels of malondialdehyde(MDA) and metallothionein(MT) at the cortex of frontal lobe in the model rats after chronic alcoholism.Method Establishment chronic alcoholic brain damage rats mode by increasing free drinking alcohol percentage gradually.Morphology changes of frontal region were observed and the level of MDA and MT in brain tissue were detected.Results In alcohol group,there were nerve cells missing,degeneration and damage,MDS content was increased and MT was decrease,compared with control group,the difference had statistical significance(P<0.01).Conclusions Chronic alcohol diet with a large amount can break the balance of oxygen radicals,increased MDA and decrease MT.