血管内皮下层纤维连接素在血小板粘附中的作用

本文利用扫描电镜和荧光分光光度计技术,研究了血管内皮下层Fn在血小板与血管壁粘附中的作用.当内皮细胞剥脱后血管壁上可见大量血小板粘附:当裸露的内皮下层失与抗Fn抗体反应后,血小板的相对粘附率降低了70.1％(P<0.01),而正常血清对照组无明显变化,说明Fn在血小板粘附中起重要作用.

The role of fibronectin in blood vessel subendothelium in platelet adhesion

The role of fibronectin in vessel subendothelium in platelet adhesion after the removal of the endothelium of aorta in normal rabbits was studied. The thoracic aortae were treated with 1% Nonidet P-40 for 5 min and with 0.3mol/L KCI for 10 min. The whole endothelium was removed and large amount of fibronectin in the subendothelium was exposed. Autogenous platelet-rich plasma was isolated and the platelets were labeled with 1 : 1000 acridine orange. The adhered platelets were washed away with 2% SDS solution. Finally, the intensity of fluorescence of the washing fluid was measured with a spectrofluorophotometer. The animals were divided into 4 groups: (1) normal endothelium group, ( 2 ) lysed endothelium group, ( 3 ) lysed endothelium and guinea pig anti-rabbit fibronectin antisera inhibition group, (4 ) lysed andothelium and guinea pig normal sera control group. The adhesion of platelets in the 2nd group,was taken as 100%. The results showed that after the treatment of anti-rabbit fibronectin antisera the platelet adhesion on subendothelium was inhibited up to 70.1%. It is suggested that the fibronectin in subendothelium plays an important role in platelet adhesion. The interaction of several kinds of macromolecular glycoprotein of vessel sudendothe-lium in platelet adhesion is discussed.