感染旋毛虫小鼠的细胞因子和T淋巴细胞亚群的动态变化

目的：探讨感染旋毛虫小鼠的细胞免疫调节作用。方法：将小鼠分为重感染组、轻感染组及对照组，于感染后不同时间随机取脾细胞制备悬液，分别以旋毛虫抗原及ＣｏｎＡ刺激后，检测白细胞介素ＩＬ－２及ＩＬ－６的活性、ＩｇＧ抗体水平以及ＣＤ４＋、ＣＤ８＋Ｔ细胞含量。结果：感染早期脾细胞以ＩＬ－２的分泌为主，而感染晚期以ＩＬ－６为主，重感染组比轻感染组的ＩＬ－２与ＩＬ－６分泌多。感染后７ｄ血清中出现ＩｇＧ抗体，２８－３５ｄ达高峰；感染后１４－２１ｄ，ＣＤ４＋含量逐渐减少，２１ｄ后又逐渐上升，而ＣＤ８＋的含量却持续增多。结论：感染４ｗｋ后，ＩＬ－６的诱生水平持续增高，可能对旋毛虫病的获得性免疫起重要作用。ＩＬ－２活性与ＣＤ８＋Ｔ细胞百分率的增高，可能提示Ｔｈ１产生的细胞因子促进ＩＬ－２活性与ＣＤ８＋Ｔ细胞活化。

OBSERVATION ON THE KINETIC CHANGES OF SPECIFIC HELPER T CELL SUBSETS OF MICE AFTER INFECTION WITH TRICHINELLA SPIRALIS

AIM: To explore the cellular immunoregulation in Trichinella spiralis infected C57BL/6J mice. METHOD: IL 2 and IL 6 levels induced by in vitro Trichinella antigen and ConA stimulated splenocytes from lightly and heavily infected C57BL/6J mice were determined by using the dependent cell line HT2 and 7TD1, IgG level was deterined by ELISA, and CD4 and CD8 levels were determined by flow cytometry. RESULTS: The production of IL 2 was predominant in the early phase after infection, while the production of IL 6 was mainly in the later phase. The levels of IL 2 and IL 6 secreted were higher in the heavily infected group than in the lightly infected group. IgG first appeared on d 7 after infection and reached its peak through d 28 to d 35 . From d 14 to d 21 the CD4 T cells showed a decrease and then increased; while the CD8 T cells increased gradually throughout the period after infection. CONCLUSION: The sustained increase in IL 6 4 weeks after infection might play an important role in the acquired immunity of trichinosis. The coincidence of the increase in IL 2 and CD8 suggests that the cytokines of Th1 might promote the activation of CD8 T cells.