Dysadherin mRNA在肾透明细胞癌中的表达及其意义

目的探讨Dysadherin在。肾透明细胞癌（RCCC）中的表达情况及其临床意义。方法选取60例新鲜RCCC组织及其癌旁肾组织。患者男35例，女25例；年龄37-78岁，中位年龄61岁；肿块直径〉7cm24例，≤7cm36例；核分级Ⅰ、Ⅱ级39例，Ⅲ、Ⅳ级21例，应用反转录．聚合酶链反应（RT-PCR）及免疫组织化学的方法分别在mRNA及蛋白水平检测Dysadherin的表达情况。结果RCCC组织中Dysadherin mRNA表达水平为2．0043±0．2890，明显高于癌旁肾组织的0．8461±0．2479（t=6．8020，P〈0．05）。Dysadherin在RCCC中的表达与肿瘤的核分级有关，其中核分级为Ⅲ、Ⅳ级的表达量明显高于Ⅰ、Ⅱ级，二者mRNA表达量分别为1．6224±0．3194、2．7780±0．2288（t=6．5750，P〈0．05），蛋白表达阳性率分别为64．1％（25／39）和95．2％（20／21）（χ^2=5．495，P〈0．05），而在患者不同性别（t=1．0530，χ^2=0．023）、年龄（t=0．0511，χ^2=0．089）、肿块直径（t=1．0330，χ^2=0．370）之间差异无统计学意义（P〉0．05）。结论Dysadherin的表达随着RCCC肿瘤细胞核分级的升高而升高，可能成为RCCC有价值的预后标志物之一。

Expression and significance of Dysadherin mRNA in renal clear cell carcinoma

Objective To investigate the expression of Dysadherin and analyze its role in renal clear cell carcinoma （RCCC）. Methods RT-PCR and immunohistochemical were used to detect the expression of Dysadherin in 60 cases of fresh RCCC and 60 adjacent normal renal tissues（male 35, female 25; age 37-78, median age 61; 〉7 cm 24, ≤7 cm 36; Ⅰ/Ⅱ 39, Ⅲ/Ⅳ21）. Results Dysadherin mRNA expression in RCCC tissues （2.0043±0.2890） was higher than that in adjacent normal renal tissues （0.8461±0.2479） （t = 6.8020, P 〈 0.05）. Dysadherin expression was associated with nuclear grade. The expression of Dysadherin in nucleus grade m and Ⅳ tumors were significantly higher than that in nucleus grade Ⅰ and Ⅱ tumors [the mRNA expression were 4.6224±0.3194, 2.7780±0.2288, the positive rates of protein were 64.1% （25/39）, 95.2 % （20/21） （t = 6.5750, χ2 = 5.495, P 〈 0.05）]. There was no association between the expression of Dysadherin with sex （t = 1.0530, χ^2 = 0.023）, age（t = 0.0511, χ^2 = 0.089） and tumor size （t = 1.0330, χ^2 = 0.370） （P 〉 0.05）. Conclusion In RCCC, Dysadherin expression is positively associated with tumor aggressiveness based on grading. It seems that Dysadherin may be a valuable prognostic marker in RCCC.