| 导流杂交基因芯片法和聚合酶链反应-反向点杂交法检测人乳头瘤状病毒亚型的比较 |
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| 引用本文： | 毛晓丹,孙蓬明,林芬,宋一一. 导流杂交基因芯片法和聚合酶链反应-反向点杂交法检测人乳头瘤状病毒亚型的比较[J]. 海峡预防医学杂志, 2011, 17(3): 4-6 |
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| 作者姓名： | 毛晓丹 孙蓬明 林芬 宋一一 |
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| 作者单位： | 福建省妇幼保健院,福州,350001 |
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| 基金项目： | 福建省医学创新课题(No.2009-CXB-33) |
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| 摘 要： | 目的 探讨导流杂交基因芯片法和聚合酶链反应-反向点杂交法检测人乳头瘤状病毒(HPV)亚型的差异.方法 随机选取HPV标本50例,分别用导流杂交基因芯片法和聚合酶链反应-反向点杂交法检测HPV亚型,比较检测结果,结果不相符或部分相符的标本采用基因测序方法验证.结果 两种方法检测结果,19例阴性标本和25例阳性标本完全相符...
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| 关 键 词： | 人乳头瘤状病毒亚型 宫颈癌 导流杂交基因芯片技术 聚合酶链式反应-反向点杂交法 |
Comparison between flow-through hybridization and gene chip and polymerase chain reaction-reverse dot blot hybridization for detecting human papilloma virus subtype |
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| MAO Xiao-dan,SUN Peng-ming,LIN Fen,et al.. Comparison between flow-through hybridization and gene chip and polymerase chain reaction-reverse dot blot hybridization for detecting human papilloma virus subtype[J]. Strait Journal of Preventive Medicine, 2011, 17(3): 4-6 |
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| Authors: | MAO Xiao-dan SUN Peng-ming LIN Fen et al. |
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| Affiliation: | MAO Xiao-dan,SUN Peng-ming*,LIN Fen,et al.Fujian Maternal and Children Hospital,350001 China |
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| Abstract: | Objection To explore the differences between flow-through hybridization and gene chip and polymerase chain reaction-reverse dot blot hybridization(PCR-RDB)for detecting human papilloma virus(HPV) subtype.Method Totally 50 samples were collected randomly to detect HPV by flow-through hybridization and gene chip and PCR-RDB method independently.Comparing the results,the different results or partly alike results were identified by genetic sequencing method.Results Totally 44 samples were coincident(19 negative... |
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| Keywords: | Human Papilloma Virus Subtype Cervical Carcinoma Flow-through Hybridization and Gene Chip Polymerase Chain Reaction-Reverse Dot Blot Hybridization(PCR-RDB) |
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