TP—ELISA法对梅毒检测的诊断学评价

目的评估梅毒螺旋体抗体酶联免疫吸附试验（Treponema pallidum antibody enzyme—linked immunosorbent assay TP—ELISA）在梅毒检测中的诊断价值。方法用梅毒螺旋体明胶凝集法（Treponema pallidnm particle agglutination test TPPA）与TP—ELISA法对60例住院及门诊梅毒患者血清和50例健康体检者血清分别进行检测，以TPPA法作为参比方法。结TP—ELISA法与TPPA法检测梅毒结果差异无显著性（P〉0．05），并且两者有极好的一致性（Kw=0．854）。TP-ELISA的诊断敏感性（Se）为90％、诊断特异性（Sp）为96．8％、阳性预告值（＋PV）为9712％、阴性预告值（－PV）为88．77％。结论TP—ELISA法与TPPA法对梅毒检测结果总体上一致，是临床珲棋的筛杏方法．适用于大批量样本检杏。

The diagnostic evaluation of TP-ELISA for detecting syphilis

Objective To evaluate the diagnosis value of TP-ELISA for detecting syphilis. Methods The serum samples from 60 patients with syphilis and 50 healthy individuals were detected by TPPA and TP-ELISA; TPPA was used as a reference test. Results There was no significant difference in the test results between TP-ELISA and TPPA (P>0.05). Two tests had excellent consistency (Kw = 0.854). The sensitivity of TP-ELISA was 90%, specificity was 96.8%, positive predictive value was 97.2%, and negative predictive value was 88.77%. Conclusions The test results of TP-ELISA for detecting syphilis are generally consistent with those of TPPA. TP-ELISA is an ideal test for clinical screening and suitable for large sample detection.