异丙酚静脉输注与颈内动脉输注对体外循环大鼠脑内氨基酸含量的影响

目的比较异丙酚颈内动脉输注与静脉内输注对中低温体外循环大鼠脑内氨基酸含量和外周循环的影响.方法建立大鼠CPB模型,随机分成5组,假手术对照组(SC),体外循环组(CPB)组,CPB+propofol2 mg/kg·h)静脉给药组(P2v组),CPB+propofol 2 mg/(kg·h)颈内动脉给药组(P2A组).结果病理检查显示,P10v组和P2A组病理损伤轻于CPB组;对脑内Glu,Asp和Gly具有抑制作用,对GABA具有促进作用.上述指标P2v组与CPB组没有差异.P10v组出现HR增快,MAP下降,而2mg/(kg·h)的异丙酚无明显的循环抑制.结论异丙酚颈内动脉连续输注具有脑保护作用,且剂量小,对循环的抑制轻微.为异丙酚脑保护研究提供新的探索.

Comparison of intracarotid and intravenous propofol for concentration of amino acids at different brain areas of rats undergoing cardiopulmonary bypass

[Objective] To study the effect of intracarotid or intravenous injection of propofol on the concentra tions of anino acids at different brain areas in rats undergoing cardiopulmonary bypass. [Methods] Fifty rats were randomly assigned to five groups. Shame group (group SC n =10); group cardiopulmonary bypass (group CPB n=10);pressure (MAP) and heart rate(HR) were monitored. Changes of the pathology were observed, and concertrations of amino acids were measured. [Results] The pathology injury of brain was attenuated in group P2A and P10v, but the pathology injury was not attenuated in group P2v. The concentrations of Glutamic acid, Asparagic acid, Glycocol, γ-Aminobutyric acid and Taurine increased undergoing CPB. The concentrations of the Glutamic acid, Asparagic acid and Glycocol dercreased after CPB in group P2A and P10v. y-Aminobutyric acid increased in group P2A and P10v, But the effect in group P2v was not significant compared with group CPB. Compared with baseline values, MAP and HR in P2v and P2A was not significantly different compared with group CPB. Compared with baseline values, MAP and HR in P2v and P2A group remained unchanged. But the MAP and HR in P10v group decreased compared with group CPB and baseline (values). [Conclusions] Intracarotid injection of propofol attenuated the injury of brain at a fraction of the intravenous dose and with less adverse systemic and cerebrovascular side effects.