| 奥曲肽抑制血管平滑肌细胞增殖及内源性IGF—I的表达 |
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| 引用本文： | 董雷,景在平,曹贵松,黄晟. 奥曲肽抑制血管平滑肌细胞增殖及内源性IGF—I的表达[J]. 解放军医学杂志, 2001, 26(9): 684-686 |
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| 作者姓名： | 董雷 景在平 曹贵松 黄晟 |
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| 作者单位： | 1. 北京军区总医院心血管外科
2. 第二军医大学长海医院, |
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| 摘 要： | 为研究生长抑素8肽类似物奥曲肽对血小板衍生生长因子AA（PDGF－AA）和胰岛素样生长因子I（IGF－I）诱导的体外培养血管平滑肌细胞（VSMCs)DNA合成、IGF－I表达的影响。采用[^3H]胸腺嘧啶核糖核苷（TdR)掺入法分析奥曲肽对PDGF－AA和IGF－I诱导VSMCs DNA合成的影响，采用免疫细胞化学法分析奥曲肽对PDGF－AA和IGF－I诱导VSMCs IGF－I表达的影响。结果发现，血清与IGF－I使VSMCs的[^3H]－TdR掺入增加，1nmol/L以上浓度的奥曲肽对此有抑制作用，且有明显的量效关系；血清和IGF－I能刺激VSMCs的IGF－I合成,10nmol/L奥曲肽与血清及IGF－I混合培养，IGF－I的灰度值分别升高16．0％和13．3％。说明奥曲肽能抑制血清和IGF－I诱导的VSMCs的DNA合成，有明显的剂量效应关系；并能抑制血清和IGF－I诱导的内源性IGF－I表达。
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| 关 键 词： | 奥曲肽 胰岛素样生长因子I 血管平滑肌细胞 内源性IGF－I |
| 修稿时间： | 2001-05-20 |
THE INHIBITORY EFFECT OF OCTREOTIDE ON THE PROLIFERATION AND EXPRESSION OF ENDOGENOUS GF-I IN VSMCs |
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| Dong Lei,Jing Zaiping,Cao Guisong et al. THE INHIBITORY EFFECT OF OCTREOTIDE ON THE PROLIFERATION AND EXPRESSION OF ENDOGENOUS GF-I IN VSMCs[J]. Medical Journal of Chinese People's Liberation Army, 2001, 26(9): 684-686 |
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| Authors: | Dong Lei Jing Zaiping Cao Guisong et al |
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| Affiliation: | Dong Lei,Jing Zaiping,Cao Guisong et al . Changhai Hospital,Second Military Medical University,Shanghai 200433 |
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| Abstract: | The effects of octreotide on DNA synthesis and the expression of endogenous insulin like growth factor I(IGF I)in newborn calf vascular smooth muscle cells(VSMCs) in vitro were analysed with 3 H thymidine incorporation and immunocytochemistry respectively.Concentration over 1 nmol/L of octreotide inhibited the increasing of 3 H thymidine incorporation induced by serum and IGF I in VSMCs. Serum and IGF I could stimulate the synthesis of endogenous IGF I in VSMCs.When cultured with 10 nmol/L of octreotide,the gray value of IGF I rose 16 0% and 13 3% respectively.It is suggested that octreotide can inhibit DNA synthesis induced by serum and IGF I in VSMCs,and the expression of endogenous IGF I in VSMCs. |
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