墓头回提取物诱导K562细胞凋亡的实验研究

目的探讨墓头回提取物体外抗白血病作用,为墓头回提取物抗肿瘤活性单体的筛选提供理论依据。方法采用MTT法测定墓头回对K562细胞的增殖抑制率,计算出IC50值;台盼蓝拒染法、流式细胞仪PI染色观察墓头回对K562细胞的促凋亡活性,用免疫细胞化学法检测墓头回对凋亡相关基因bcl-2和bax蛋白表达的影响。结果墓头回对K562细胞增殖具有明显的抑制作用,且在一定的剂量范围内其抑制作用具有明显的剂量依赖性,IC50为36.03 mg/L。墓头回作用于K562细胞后,倒置显微镜下可见典型的凋亡细胞的形态学特征。流式细胞仪检测结果显示,实验组细胞凋亡率明显高于对照组,与对照组相比差异显著(P<0.05)。结论墓头回作用于K562细胞,可以明显抑制K652细胞增殖。此外,诱导K562细胞凋亡可能是其发挥抗肿瘤作用的机制之一。

Inductive effect of diversifolious patrinia root extract on K562 cells apoptosis

objective to investigate the effects and mechanism of diversifolious patrinia root(DPR) extract on proliferation and apoptosis of K562 cells to offer experimental data for screening bioactive components from DPR.Methods MTT method was used to detect inhibition ratio to K562 proliferation and IC50.The morphological characteristics of apoptosis cells were observed by trypan blue stain.The apoptosis ratio and cell cycle of K562 were measured by flow cytometry(FCM).The expression of apoptosis related protein bcl-2 and bax were determined by immunocytochemistry.Results DRP extract obviously inhibited the proliferation of K562 cells in a does-dependent manner in the definite dose range,and the IC50 was 36.03 mg/L.The typical morphological characteristics of apoptosis cells could be seen under the inverted microscope.The apoptosis rates in the treatment groups were significant higher than that in the control group(p<0.05).The expression of bcl-2 protein was down-regulated,and the expression of bax protein was up-regulated after K562 cells were treated by DRP extract for 48 h,and the differences between the treatment group and the control group were significant(p<0.05).Conclusion DRP extract can inhibit the proliferation and induce the apoptosis of K562 cells,which may be one of the mechanisms of anti-tumor effect of it.