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复方血尿停经肝微粒体代谢后对肾小球系膜细胞的影响
引用本文:张红敏 丁樱 陈世伟 王丽英. 复方血尿停经肝微粒体代谢后对肾小球系膜细胞的影响[J]. 医学教育探索, 2005, 0(2): 233-236
作者姓名:张红敏 丁樱 陈世伟 王丽英
作者单位:成都中医药大学,河南中医学院第一附属医院,四川大学华西公共卫生学院营养与食品卫生教研室,成都中医药大学 四川成都610075,河南郑州450008,四川成都610041河南省疾病预防控制中心,河南郑州450003,四川成都610075
基金项目:河南省自然科学基金资助项目(0111023200)
摘    要:目的探讨复方血尿停治疗以系膜增生为主要病理改变的肾小球疾病的作用机制。方法将复方血尿停与大鼠肝微粒体共同孵育后加入到肾小球系膜细胞(GMC)体外培养体系中,MTT法观察细胞增殖情况,放射免疫法测定白细胞介素-6(IL-6)、内皮素-1(ET-1)水平,速率法检测乳酸脱氢酶(LDH)的含量。结果经肝微粒体代谢后,复方血尿停2、4、8mg/mL均抑制GMC的增殖及IL-6、ET-1的产生,且呈剂量依赖方式。结论复方血尿停能抑制IL-6、ET-1的分泌和GMC的增殖,此作用可能是其治疗系膜增生性肾小球疾病的作用机制之一。

关 键 词:复方血尿停  肾小球系膜细胞  增殖  白细胞介素-6(IL-6)  内皮素-1(ET-1)

Effects of Compound Xueniaoting metabolized by liver microsomes on glomerular mesangial cells
ZHANG Hong-min,DING Ying,CHEN Shi-wei,WANG Li-ying. Effects of Compound Xueniaoting metabolized by liver microsomes on glomerular mesangial cells[J]. Researches in Medical Education, 2005, 0(2): 233-236
Authors:ZHANG Hong-min  DING Ying  CHEN Shi-wei  WANG Li-ying
Abstract:Objective To study the mechanism of traditional Chinese medicine Compound Xueniaoting (CXNT) in treating glomerulus diseases with the main pathological changes of proliferation in mesangial cells. Methods CXNT and rat liver microsomes were incubated together, and then the incubated CXNT was added into cultured glomerular mesangial cells (GMC) in vitro. The proliferation of GMC was observed by MTT assay, the levels of interleukin-6 (IL-6) and endothelin-1 (ET-1) were determined by radioimmunoassay, and content of lactic dehydrogenase (LDH) was determined by velocity assay. ResultsCXNT (2, 4, 8 mg/mL) metobolized by rat liver microsomes could all inhibite the proliferation of GMC and production of IL-6 and ET-1 in a dose-dependent manner. Conclusion CXNT can inhibite the proliferation of GMC and restrain the secretion of IL-6 and ET-1, this function may be one of CXNT mechanisms in treating some mesangial proliferative glomerulonephritis.
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