| HPLC测定小儿腹泻外敷散中吴茱萸次碱的含量 |
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| 引用本文： | 冯果,刘文,冯勇.HPLC测定小儿腹泻外敷散中吴茱萸次碱的含量[J].微量元素与健康研究,2011,28(5):40-42. |
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| 作者姓名： | 冯果 刘文 冯勇 |
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| 作者单位： | 1. 贵阳中医学院,贵阳,550002
2. 威宁县人民医院,贵州威宁,553100 |
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| 基金项目： | 贵州省卫生厅项目(gzwkj2010-1-45) |
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| 摘 要： | 目的:建立小儿腹泻外敷散中吴茱萸次碱含量的测定方法。方法:采用高效液相色谱法测定小儿腹泻外敷散中吴茱萸次碱含量。色谱柱:20RBAX Eclipse XDB-Cl8(4.6 mm×250 mm,5μm);流动相:乙腈-水-四氢呋喃-冰醋酸(6∶5∶0.9∶0.1);检测波长:345 nm;流速:1.0 ml/min;进样量:10μl;柱温:30℃。结果:吴茱萸次碱在0.292~2.920μg(r=0.999 4)范围内线性关系良好。回归方程为:Y=29 627X+3 524.3,r=0.9994,吴茱萸次碱的平均回收率为99.66%,RSD为0.95%。结论:方法简便可行、重复性好,可作为小儿腹泻外敷散中吴茱萸次碱的含量测定方法。
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| 关 键 词： | HPLC 小儿腹泻外敷散 吴茱萸次碱 含量测定 |
Content determination of Rutaecarpine in Xiao'er Fu Xie Wai Fu Powder by HPLC |
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| FENG Guo,LIU Wen,FENG Yong.Content determination of Rutaecarpine in Xiao'er Fu Xie Wai Fu Powder by HPLC[J].Studies of Trace Elements and Health,2011,28(5):40-42. |
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| Authors: | FENG Guo LIU Wen FENG Yong |
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| Institution: | FENG Guo1,LIU Wen1,FENG Yong2(1.Gui Yang College of Traditional Chinese Medicine,Gui Zhou Gui Yang 550002,2.Weining Autonomous County People's Hospital,Gui Zhou Weining 553100,China) |
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| Abstract: | Objective:To establish a method for determing Rutaecarpine in Xiao’er Fu Xie Wai Fu Powder.Methods: HPLC deternination of Rutaecarpine in decoction of Xiao’er Fu Xie Wai Fu Powder.Column: 20RBAX Eclipse XDB-Cl8(4.6 mm×250 mm,5 μm);mobile phase:acetonitrile-water-THF-acetic acid (6∶5∶0.9∶0.1);detection wavelength: 345 nm;flow rate: 1.0 ml/min;injection volume: 10 μl;column temperature: 30 ℃.Results:Rutaecarpine in 0.292～2.920 μg(r=0.999 4) a good linear relationship.Regression equation:Y=29627X +3524.3,r=0.9994,rutaecarpine average recovery was 99.66%,RSD 0.95%.Conclusions:The method is simple and reproducible for the determination of Rutaecarpine in decoction of Xiao’er Fu Xie Wai Fu Powder by HPLC. |
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| Keywords: | HPLC Xiao'er Fu Xie Wai Fu Powder Rutaecarpine Determination |
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