| 葡萄糖酸铬对体外高糖培养乳鼠心肌细胞保护作用 |
| |
| 引用本文： | 张杰,杨旭东,王春涛.葡萄糖酸铬对体外高糖培养乳鼠心肌细胞保护作用[J].微量元素与健康研究,2011,28(5):4-5. |
| |
| 作者姓名： | 张杰 杨旭东 王春涛 |
| |
| 作者单位： | 牡丹江医学院,黑龙江牡丹江,157011 |
| |
| 基金项目： | 治疗胰岛素抵抗中草药筛选评价系统的建立及新药寻找(11511427) |
| |
| 摘 要： | 目的:探讨葡萄糖酸铬对体外高糖培养乳鼠心肌细胞的保护作用及其机制。方法:Wistar乳鼠原代细胞随机分为空白对照组、高糖组及葡萄糖酸铬高、低剂量组。用荧光显微镜测定各组细胞凋亡情况。结果:经葡萄糖酸铬干预后,心肌细胞存活率明显增高,凋亡细胞明显减少,bcl-2表达增加,caspase-3表达减少。结论:葡萄糖酸铬抑制体外高糖培养的心肌细胞凋亡,增高细胞存活率,其机制可能与抑制caspase-3、激活bcl-2表达有关。
|
| 关 键 词： | 铬 糖尿病 细胞凋亡 bcl-2 caspase-3 |
The Protect Effect of chromium gluconant on the cardiomyocytes in cultured neonate rat in glucose |
| |
| ZHANG Jie,YANG Xu-dong,WANG Chun-tao.The Protect Effect of chromium gluconant on the cardiomyocytes in cultured neonate rat in glucose[J].Studies of Trace Elements and Health,2011,28(5):4-5. |
| |
| Authors: | ZHANG Jie YANG Xu-dong WANG Chun-tao |
| |
| Institution: | ZHANG Jie,YANG Xu-dong,WANG Chun-tao(Mudanjiang Medical College,Heilongjiang Mudanjiang 157011,China) |
| |
| Abstract: | Objective:To investigate the effect of chromium gluconant on glucose on the cardiomyocytes apopotosis in cultured neonate rat and its mechanism.Methods: Primitive cardiomyocytes of Wistar neonate rats were randomly divided into normal control group,the glucose group,0.1 mg/mL chromium gluconant,0.2 mg/mL chromium gluconant.Cell apoptosis was measured by fluorescence microscope and the levels of bcl-2 and caspase-3 gene expression were measured by RT-PCR.Results:chromium gluconant could inhibite the apoptosi... |
| |
| Keywords: | chromium diabetes Cell apoptosis bcl-2 caspase-3 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
