长期低剂量接触甲基对硫磷的效应生物标志物研究

目的 探讨长期低剂量接触甲基对硫磷（M1605）的效应生物标志物。方法 选择某农药厂M1605合成和包装车间共71名作业工人为研究对象，同厂不接触毒物的健康工人50名为对照组。检测其全血乙酰胆碱酯酶活性（bl-AChE），神经行为功能，末梢血T淋巴细胞亚群，血清中IgG、IgA、IgM，补体C3、C4含量和内分泌（FSH、LH、T）水平。结果 作业工人没有明显的症状和体征，全血bl-AChE活性显著降低，神经行为功能中平均反应时显著延长，数字译码速度明显减慢，视觉记忆能力显著降低，T淋巴细胞亚群中CD4、CD4/CD8都显著降低。而血清免疫球蛋白IgG、IgA、IgM，补体C3、C4含量移山倒海分泌FSH、LH、T皆无明显的变化。结论：初步认为bl-AChE、神经行为功能和T淋巴细胞亚群的变化可考虑作为长期低剂量接触M1605的效应生物标志物，尤其CD4/CD8指标可作为其早期免疫毒性效应生物标志物。

Investigation on the effect biomarkers of long term and low level exposure to methylparathion

Objectives This study aims at the effect biomarkers of long term and low level exposure to methylparathion(M1605).Methods Seventy one workers exposed to M1605 from a large pesticide manufacturing plant were selected as subjects,while 50 non exposed healthy workers from the same factory served as controls.Blood samples were collected for measuring the activity of the whole blood acetylcholinesterase(bl AChE),T lymphocyte subpopulation,concentration of serum IgG,IgA,IgM,C 3,C 4 and hormone levelfollicle stimulating hormone(FSH),luteinizing hormone(LH) and testosterone(T)].Symptom checklist(SCL 90),physical examination,and several subtests of neurobehavioral core test battery (NCTB) were also applied in this study.Results There was no any obvious symptom or sign in all subjects.In comparison with the control group,the exposed workers had lower activity of bl AChE,reduced T lymphocyte subpopulation(CD 4,CD 4/CD 8),and worse performance in several subtest of NCTB,such as,mean reaction time,digital coding,and visual retention,but no change in serum IgG,IgA,IgM,C 3,C 4,FSH,LH and T.Conclusion The changes of bl AChE,T lymphocyte subpopulation,and neurobehavioral function could be taken as the early effect biomarkers of low level exposure to M1605.