胆管癌组织端粒酶活性检测及意义

目的　探讨端粒酶活性与胆管癌的关系及其诊断意义。方法　采用端粒酶PCR ELISA法检测 2 3例胆管癌组织的端粒酶活性 ,同时以 5例胆管癌癌旁组织及 5例正常胆总管组织做对照。结果　 2 3例胆管癌组织中有 1 8例显示端粒酶活性(78.3% ) ,而癌旁组织、正常胆总管组织未显示活性。端粒酶阳性检出率与患者性别、肿瘤部位、分化程度、大小等因素无相关性 ,但肿瘤转移与端粒酶阳性检出率有显著差异 (P <0 .0 5 )。结论　端粒酶见于大多数胆管癌组织中 ,其活性可能在胆管癌的发生发展中起重要作用 ,有望成为恶性肿瘤诊断的标记物

Determination of telomerase activity in carcinoma of bile duct and its clinical significance

Objective To explore the relationship between telomerase activity and bile duct carcinoma and its significance in clinical diagnosis. Methods Telomerase activities were examined in 23 cases of bile duct carcinoma, 5 cases of carcinoma adjacent tissues and 5 cases of normal bile duct tissues respectively with telomerase PCR ELISA method. Results Telomerase activity was detected in 18 of 23 cases (78.3%) of bile duct carcinoma, and was not found in 5 cases of carcinoma adjacent tissues and 5 cases of normal bile duct tissues. The detection rate of telomerase activity had no correlation with patients' age, sex, tumor site and size but significantly correlated to metastasis of tumor ( P <0.05). Conclusion The level of telomerase activity was significantly higher in bile duct carcinoma and may be served as one of the clinical marker for malignant neoplasm because of its spsecificity.