| 星点设计-效应面法优化川西獐牙菜提取工艺 |
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| 引用本文： | 邹小艳,魏立新,杜玉枝,郭松长,韩青,肖远灿.星点设计-效应面法优化川西獐牙菜提取工艺[J].中草药,2008,39(5):692-696. |
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| 作者姓名： | 邹小艳 魏立新 杜玉枝 郭松长 韩青 肖远灿 |
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| 作者单位： | 中国科学院西北高原生物研究所, 青海西宁 810008;中国科学院研究生院, 北京 100049;青海省青藏高原特色生物资源研究重点实验室, 青海西宁 810008;中国科学院西北高原生物研究所, 青海西宁 810008;青海省青藏高原特色生物资源研究重点实验室, 青海西宁 810008;中国科学院西北高原生物研究所, 青海西宁 810008;青海省青藏高原特色生物资源研究重点实验室, 青海西宁 810008;中国科学院西北高原生物研究所, 青海西宁 810008;中国科学院西北高原生物研究所, 青海西宁 810008;中国科学院研究生院, 北京 100049;青海省青藏高原特色生物资源研究重点实验室, 青海西宁 810008;中国科学院西北高原生物研究所, 青海西宁 810008;青海省青藏高原特色生物资源研究重点实验室, 青海西宁 810008 |
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| 摘 要： | 目的 星点设计-效应面法优化川西獐牙菜的提取工艺.方法 以乙醇体积分数、提取时间、溶媒比为自变量,龙胆苦苷收率和浸膏得率为因变量,通过对自变量各水平的多元线性回归及二项式拟合,用效应面法选取较佳工艺,并进行预测分析.结果 确定最优提取工艺为86%乙醇12.4倍量回流提取3次,每次63 min.结论 星点设计-效应面法优选的川西獐牙菜提取工艺,方法简便,精度更高.
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| 关 键 词： | 川西獐牙菜 星点设计 效应面法 龙胆苦苷 浸膏 星点设计 效应面法 优化 川西獐牙菜 提取工艺 central composite design process Optimum method surface 精度 优选 回流提取 倍量 最优 结果 预测分析 较佳工艺 选取 拟合 |
| 收稿时间： | 2007/9/14 0:00:00 |
Optimum extracting process for Swertia mussotii by central composite design/response surface method |
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| ZOU Xiao-yan,WEI Li-xin,DU Yu-zhi,GUO Song-chang,HAN Qing and XIAO Yuan-can.Optimum extracting process for Swertia mussotii by central composite design/response surface method[J].Chinese Traditional and Herbal Drugs,2008,39(5):692-696. |
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| Authors: | ZOU Xiao-yan WEI Li-xin DU Yu-zhi GUO Song-chang HAN Qing and XIAO Yuan-can |
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| Institution: | Northwest Institute of Plateau Biology,Chinese Academy of Sciences, Xining 810008,China;Graduate University,Chinese Academy of Sciences,Beijing 100049,China;Qinghai Key Laboratory of Qinghai-TibetPlateau Biological Resources,Xining 810008,China;Northwest Institute of Plateau Biology,Chinese Academy of Sciences, Xining 810008,China;Qinghai Key Laboratory of Qinghai-TibetPlateau Biological Resources,Xining 810008,China;Northwest Institute of Plateau Biology,Chinese Academy of Sciences, Xining 810008,China;Qinghai Key Laboratory of Qinghai-TibetPlateau Biological Resources,Xining 810008,China;Northwest Institute of Plateau Biology,Chinese Academy of Sciences, Xining 810008,China;Northwest Institute of Plateau Biology,Chinese Academy of Sciences, Xining 810008,China;Graduate University,Chinese Academy of Sciences,Beijing 100049,China;Qinghai Key Laboratory of Qinghai-TibetPlateau Biological Resources,Xining 810008,China;Northwest Institute of Plateau Biology,Chinese Academy of Sciences, Xining 810008,China;Qinghai Key Laboratory of Qinghai-TibetPlateau Biological Resources,Xining 810008,China |
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| Abstract: | Objective To optimize the extracting process for Swertia mussotii by central composite design/response surface method.Methods Independent variables were ethanol concentration,reflux time,and solvent ratio,dependent variables were extracting rate of gentipicroside and extract.Central composite design and response surface method were used to optimize the extracting process,A two-order polynomial equation was fitted to the data and resultant model was used to predict the response in the optimal region.Results Analysis indicated the optimum conditions were 86% ethanol,63 min for once reflux, 12.4-fold solvent and 3 times for extraction.Conclusion It shows that the optimum precess is simple and more convenient for extracting S.mussori,with an higher precision. |
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| Keywords: | Swertia mussotii Franch central composite design response surface method gentipicroside extract |
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