黄芪丹参复方提取物对一氧化氮合成阻滞孕鼠胎盘血管内皮细胞及滋养细胞血管内皮生长因子mRNA表达影响

目的 研究黄芪丹参复方提取物提高胎盘供血的分子机制，为临床有效防治胎盘供血不足所致妊娠并发症提供思路。方法 提取分离中药黄芪、丹参复方成分，运用一氧化氮合酶(NOS)阻滞剂L-精氨酸甲酯(L-NAME)建立一氧化氮合成阻滞大鼠模型，核酸原位杂交技术检测妊娠12日胎盘滋养细胞、始基微血管内皮细胞血管内皮生长因子(VEGF)mRNA表达变化。结果 一氧化氮合成阻滞模型VEGF mRNA表达较空白组、中药治疗组及硝酸甘油治疗对照组均明显降低(P<0．05，P<0．01)。结论 黄芪丹参复方成分可能对妊娠早期胎盘滋养细胞及血管内皮细胞VEGF mRNA表达具有促进作用，从而有利于胎盘血管网络形成及滋养细胞侵入及母胎循环构建，提高胎盘血液供应，达到防治妊娠高血压综合征等胎盘供血不足疾病的目的。

Effect of Ingredients of Astragalus Salvia Compound on Vascular Endothelial Cell in Placenta and Vascular Endothelial Growth Factor mRNA Expression in Trophocyte in Pregnant Rats with Inhibited Nitric Oxide Synthesis

ObjectiveTo investigate the molecular mechanisms of effective ingredients of Astragalus-Salvia compound (ASC) in increasing placental blood supply, to provide thought for establishing an effective prevention and treatment of insufficient placental blood supply caused complications of pregnancy. MethodsThe effective ingredients of ASC was isolated and abstracted and the rat model of inhibited nitric oxide synthesis was established by using L-Arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor. The mRNA expression of vascular endothelial growth factor (VEGF) in placental trophocyte and primordial micrangial endothelial cell was determined by using in situ hybridization at 12th day of pregnancy. ResultsThe VEGF mRNA expression was significantly lower in nitric oxide synthesis inhibited model rats than that in rats non-modeled, or in model rats treated by ASC abstracts or nitroglycerin (P<0.05,P<0.01). ConclusionEffective ingredients of ASC may improve VEGF mRNA expression in placental trophocyte and vascular endothelial cell in early pregnancy, and thus be favorable for placental vascular net formation,trophocyte infiltration and mother-placental circulation constitution for increasing placental blood supply to prevent and treat the insufficient placental blood supply caused diseases such as pregnancy-induced hypertension syndrome.