Lectin typing of methicillin-resistant Staphylococcus aureus |
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Authors: | Muñoz A Alvarez O Alonso B Llovo J |
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Affiliation: | Department of Microbiology and Parasitology, Faculty of Biology, University of Santiago de Compostela, Spain. mpangels@uscmail.usc.es |
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Abstract: | This study reports the patterns of agglutination of 77 clinical isolates of methicillin-resistant Staphylococcus aureus by 32 commercially available lectins. Cell suspensions were not pre-treated. Each isolate was cultured on three media: Columbia blood agar, trypticase-soy agar and Chapman Stone agar. The lectins agglutinating each isolate varied widely depending on culture medium; only five isolates were agglutinated by the same set of lectins regardless of the culture medium used. Lectin typing could be a useful epidemiological tool, but it is necessary to standardise assay conditions (notably culture medium) to enable meaningful comparison of the results produced by different research groups or centres. |
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