核黄素和抗坏血酸对脱氧雪腐镰刀菌烯醇诱导小鼠胸腺细胞凋亡和增殖抑制影响的研究

目的:探讨核黄素和抗坏血酸在体内对脱氧雪腐镰刀菌烯醇 (DON)诱发小鼠胸腺细胞凋亡的阻断作用。方法:采用动物实验,应用DNA琼脂糖凝胶电泳和流式细胞术检测方法分析核黄素和抗坏血酸预处理对DON诱导小鼠胸腺细胞凋亡和增殖抑制的影响。结果:FCM检测结果表明,4mg/kgDON可诱导小鼠胸腺细胞凋亡,其凋亡率为13.73%±1.53 %。核黄素 (1.25-10mg/kg)和抗坏血酸 (25-100mg/kg)预处理组小鼠胸腺细胞凋亡率均明显低于DON组 (P <0.05),但不同剂量核黄素和抗坏血酸预处理组间凋亡率无明显差异。DNA琼脂糖凝胶电泳结果亦证实,DON处理组细胞出现明显的凋亡特有的“梯状条带”,而核黄素和抗坏血酸预处理各组及生理盐水对照组细胞均未出现“梯状条带”。给予核黄素和抗坏血酸预处理的各DON处理组细胞增殖指数与DON处理组细胞增殖指数之间无明显差异。结论:核黄素和抗坏血酸均可一定程度抑制DON体内对小鼠胸腺细胞的致凋亡作用,但对DON抑制胸腺细胞增殖的作用无明显影响.

Effects of riboflavin and ascorbic acid on apoptosis and proliferative inhibition of mouse thymocytes induced by deoxynivalenol in vivo

AIM: To explore the effects of riboflavin and ascorbic acid on the apoptosis induced by deoxynivalenol(DON) in mouse thymocytes. METHODS: The effects of riboflavin and ascorbic acid on the apoptosis and proliferation inhibition of thymocytes induced by DON in KM mice were studied with animal experiment, DNA agarose gel electrophoresis and flow cytometric DNA content analysis. RESULTS: Apoptosis rate of thymocytes in DON (4 mg/kg) treated group was13.73%±15.3% The percentages of apoptosis in riboflavin (1.25 mg/kg-10mg/kg) and ascorbic acid (25 mg/kg-100mg/kg) pretreated thymocytes groups were significantly lower than that in DON group (P <0.05). The result of DNA agarose gel electrophoresis showed that the characteristic ladder pattern of apoptosis was found in DON-treated thymocytes, but not in control and riboflavin pretreatment and ascorbic acid pretreatment groups The significant differences in proliferation index were not found among DON-treated thymocytes and riboflavin and ascorbic acid-pretreated thymocytes CONCLUSION: Pretreatment with riboflavin and ascorbic acid inhibit apoptosis of mouse thymocytes induced by DON in certain extent and have no effect on proliferation inhibition by DON.